Abbreviations: Adeno 5, adenovirus C subtype 5; BGM, buffalo-green-monkey cells; CA9, coxsackievirus subtype 9; CPE, cytopathogenic
effect; EC50, effective concentration 50; FluA, influenza A virus; FluB, influenza B vims; HEp-2, human epithelial cells; HRV14, human rhinovirus B subtype 14; IC50, inhibitory concentration 50; MDCK, Madin-Darby-Canine-Kidney cells; M.
31 EID50 was inoculated and the cell culture flasks were incubated at 37oC in the presence of 5% CO2 in a CO2 incubator and observed for cytopathogenic
After occurrence of a cytopathogenic
effect in a culture, the supernatant was harvested and stored at -20[degrees]C for DNA extraction.
To exclude presence of non-EV-71 strains in our virus stocks that caused the cytopathogenic
effect in the neutralization assays, we plaque-purified viruses incubated with or without the polyclonal serum from rabbits and a selection of the IVIg batches.
No viruses were indicated by the cytopathogenic
evidence or by the haemadsorption test.
This could be ascertained with different assays as plaque-reduction assays in plaque forming units (PFU), the analyses of a cytopathogenic
effect (CPE) and with enzyme immunoassays (EL1SA) to determine the amount of newly synthesised virus.
It has worldwide distribution and exits as a cytopathogenic
(CP) and noncytopathogenic (NCP) biotype, based on the presence or absence of visible cytopathic effects in the cell culture (Dubovi, 1990).
Rearrangement of viral sequences in cytopathogenic
HIV-1 glycoprotein 120 induces the MMP-9 cytopathogenic
factor production that is abolished by inhibition of the p38 mitogen-activated protein kinase signaling pathway.
Second, supernatants from the virus isolate from South Korea showed high cytopathogenic
activity in cell culture (103-105 cytopathogenic
units/mL) but low levels of concomitant viral RNA by RT-PCR.
Cells in each well were examined for the presence of any cytopathogenic
(CPE) effects which formed the bases of TCID50 calculation of the virus following formulation as described by Villegas (1988).
The TCID50 (tissue culture infection dose) of HSV-II towards Vero cells, determined by cytopathogenic
effect (mPE), was [10.