Assays of antiviral activity and cytotoxicity were evaluated by the SRB method using cytopathic
effect (CPE) reduction recently reported (Choi et al.
Cells of the monocyte/macrophage lineage represent the other major target of HIV infection, but these cells are relatively unaffected by HIV's cytopathic
The infected RD cells were observed for the cytopathic
effect (CPE) or harvested at eight-hour intervals post infection to determine the number of viral RNA copies by quantitative RT-PCR (qRT-PCR).
They showed that minocycline (and doxycycline) prevented HIV-mediated cytopathic
effects in vitro, 7-14 days post-infection.
The diagnosis of CMV is established by the identification of viral cytopathic
effect (intranuclear inclusions) in gastrointestinal mucosal biopsies by routine haematoxylin-eosin (H&E) stains .
Instead, histopathology of specimens taken from the left piriform sinus, left aryepiglottic fold, left vallecula, and left tonsillar pillar demonstrated a viral cytopathic
effect and necrosis consistent with HSV infection.
The inoculated cultures were incubated for 120 hours at 27[degrees]C and examined for signs of cytopathic
effect (CPE) due to baculovirus.
At that time, the most rapid diagnosis was by production of cytopathic
effect after subpassage in tissue culture (7), but most coronaviruses were recognized only with the use of human embryo tracheal organ cultures, and viral presence was detected by cessation of ciliary motion, by electron microscopy of nutrient media, or most cumbersome of all, by inoculation and production of colds in human volunteers (8, 9).
No viral cytopathic
effect was noted in this specimen.
effects (CPE) in CEF are characterized by rounding of the cells followed by degenerative changes and necrosis.
This virus isolate produced cytopathic
effects in chicken embryo liver (CEL) cells and reacted with fowl adenovirus 4 (FadV-4) antiserum in agar gel immunodiffusion and immunofluorescence tests.
As the authors note, evidence exists suggesting that cold symptoms might be related to inflammatory mediators such as prostaglandins and kinins, which are blocked by NSAIDs, rather than to direct viral cytopathic