Ex vivo cytokine assay
demonstrated that the combined immunization significantly upregulated the secretion of IFN-[gamma] and conversely downregulated the secretion of IL-4, IL-10, and TGF-[beta] in the splenocyte population compared to the splenocytes from the control (vehicle-treated) tumor-bearing mice (figure 3).
While this is the first cytokine assay
in the STELLUX[R] Chemiluminescence product line, the new assay still offers a broad dynamic range similar to other STELLUX[R] kits.
A MOI of 10:1 was used for the cytokine assay
to ensure sufficient cytokine production.
The results of cytokine assay
confirmed the ability of PapG.
2] atmosphere for 24 hours, supernatant was collected for cytokine assay
The cytokine assay
was done using IL-10 serum levels which were detected using ELISA technique (eBioscience, ESP), immediately after blood collection.
for TNF-[alpha], INF-[gamma], IL-1[beta] and IL-10 was done by ELISA kit method (Diaclone, France).
A drop of blood was used to make blood smears for malaria diagnosis while the rest of the blood was centrifuged and plasma separated and stored at -20oC (Webuye Sub-District Hospital) and later at -70oC (Moi Teaching and referral Hospital) until required for cytokine assay
Placental markers were measured by immunohistochemistry, and cord blood cytokines by multiplex cytokine assay
IL-1/3 and TNF-[alpha] were assayed by using the custom-coated MSD 96-well Multi-Array[R] Human Cytokine Assay
Ultrasensitive kit (Meso Scale Discovery, cat #N41IB-1) according to the manufacturer's kit instructions for serum/plasma samples.
Our results also indicated that RT-PCR was more sensitive than ELISA for the cytokine assay
After centrifugation of the blood at 3000 rpm for 10 minutes at 4[degrees]C, separated plasma samples were stored in two or three aliquots (120[micro]l) at -70[degrees]C until cytokine assay