trituberculatus share the same heme-binding motif region, three histidine-rich regions (HXXXH, HXX(X)HH, and Q/HXXHH), and the cytochrome b5
However, the activities of cytochrome b5
(CB) and N.
Total microsomal CYP and cytochrome b5
were measured according to the method of Omura and Sato, 1964 , using the molar extinction coefficients 91 and 185 m[M.
When SARA was induced, acyl-CoA synthetase (ACS) and cytochrome b5
involved in lipid metabolism were up-regulated, phosphoenolpyruvate carboxykinase (PEPCK) involved in glucose metabolism was downregulated, while enolase was up-regulated.
Congenital or inherited causes of methemoglobinemia result due to decreased activity of the enzyme cytochrome b5
reductase resulting in diminished enzymatic reduction of the hemoglobin molecule.
3]) by losing electron is prevented by cytochrome b5
system of the body and normally only 1-2% of hemoglobin is present in ferric state in the body.
reductase deficiency and mental retardation.
Hereditary methemoglobinemia, characterized by a deficiency of NADH-dependent cytochrome b5
reductase, has a wide geographic distribution (2).
The description of the inheritance of congenital cytochrome b5
reductase deficiency as an autosomal recessive condition in the context of the father and two sons being clinically affected is unusual but possible.
The reaction mixture with CYP3A4/OR or CYP3A5/OR was additionally supplemented with purified cytochrome b5
(P450:b5 ratio, 1:1) according to a previously described method (26) before the start of the reaction with NADPH.
reductase activity could not be measured in the neonates because of the lack of technical facilities.
Comparisons of the cytochrome b5
-like domain with cytochrome b5
are shown in Figure 3.