inhibition of Sonic hedgehog signal transduction is not mediated through effects on cholesterol transport.
, an inhibitor of Hedgehog, reduced tumor mass of xenografted invasive pancreatic cancers in nude mice.
Phytoalkaloids like cyclopamine
are present in a range of plants and result in severe birth defects of offspring when pregnant animals are exposed to them.
, topical tazarotene, and topical antioxidants all hold promise, said Dr.
decreased cell number by half and decreased the percentage of late erythrocytes on each harvest day.
Cell lines were prepared from the yolk sac and exposed to Cyclopamine
for one, two, and three days.
In recent years, scientists have tested cyclopamine
to see whether its assault on the fast-growing cells of embryos and fetuses might be turned against tumor cells, which also multiply rapidly.
The hedge hog pathway drives the proliferation of cancer stem cells and has been a target for drug development for sometime, based on earlier work with the plant-derived substance, cyclopamine
, a specific blocking agent of the hedge hog pathway.
This research opens the possibility of new anti-cancer therapies, specifically the use of RNA interference and of Cyclopamine
, a plant product known to block Hedgehog pathway activity.
Moreover, inhibition of Hedgehog signaling with the small molecular cyclopamine
caused a profound decrease in metastatic spread in this orthotopic xenograft model.
Using small-scale microarray technology, a panel of 96 cell cycle control genes was screened for changes in expression in the presence of cyclopamine
The report states that administration of a Hedgehog pathway inhibitor, cyclopamine
, blocks the growth of the breast cancer cells that were isolated from those tumor tissues.