sensitivity as a taxonomic character in Ceratocystis.
Further, 488 Penicillium/Cladosporium strains were recovered from cycloheximide
and 407 from cycloheximide
-free Petri dish cultures.
Consistent with this, inhibition of protein synthesis by cycloheximide
significantly decreases the accumulation of ubiquitinated proteins and blocks falcarindiol induced endoplasmic reticulum stress and cell death.
During January 2008-December 2010, we screened 3,186 fecal samples on brain-heart infusion agar with 5% defibrinated sheep blood and antimicrobial drugs (bacitracin, cycloheximide
, colistin sulfate, cephazoline sodium, novobiocin) and incubated under microaerophillic environment (5% [O.
embryos (n = 3) rescued for atRA signaling through the repeated administration of low, nonteratogenic doses of atRA (Figure 5A), To further assess the effect of atRA on Ahr expression, we examined the level of Ahr mRNA in WT MEFs cultured with cycloheximide
, an inhibitor of protein translation, and found a 5-fold increase upon atRA stimulation (Figure 5B).
Collected brush samples were immediately inoculated onto modified Dixon's agar (MDA), as a selective media containing cycloheximide
To determine the half-life of telomerase activity, K562 and HeLa cells were treated with 300 mg/L cycloheximide
(CHX; Sigma), a protein synthesis inhibitor, for up to 48 h, as described by Holt and co-workers (5, 6).
To prevent overgrowth and a resultant reduced sensitivity to Chlamydia attachment, the supplier adds cycloheximide
to the monolayers, which slows cell growth.
At doses of similar toxicity, cycloheximide
affects a larger number of transcription factors than HHT, and the effects of HHT on transcription factors appear to be more specific.
Amphotericin B, canthaxanthin, calcein-AM (CAM), cisplatin, crocin, cycloheximide
, doxorubicin, etoposide, [beta]-carotene, fucoxanthin, paclitaxel, vinblastine, 5-fluorouracil (5-FU), retinoic acid, rhodamine 123 (Rho123), and 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyl-tetrazolium bromide (MIT) were purchased from Sigma-Aldrich[R] GmbH, Germany.
, and incubated at 25[degrees]C or 35[degrees]C for as long as 6 weeks (10).
After inhibiting protein synthesis using cycloheximide
(CHX), we found that levels of both megalin and CLC5 were lower in Cd-challenged cells than in cells treated with Cd or CHX only, which is consistent with reduced translation and/or posttranslational down-regulation.