CCNB1

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CCNB1

A gene on chromosome 5q12 that encodes cyclin B1, which is essential for controlling the cell cycle at the G2/M transition. Cyclin B1 interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex, the maturation promoting factor, and interacts with RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase.
References in periodicals archive ?
2]/M phases and affected cyclin B1, Bcl-2, and Ki67 expression.
Antibodies against Bach1 (sc-14700), Bcl2 (sc-7382), Bcl-xL (sc-8392), HO-1 (sc-1796), cyclin D1 (sc-20044), cyclin E2 (sc-28351), cyclin B1 (sc-595), CDK2 (sc-163), CDK4 (sc-601), CDK6 (sc-177), p21 (sc-397), p53 (sc-126), the human Bach1 siRNA (sc-37064), and the control siRNA were obtained from Santa Cruz Biotechnology (Santa Cruz, CA).
7 cells through cell cycle arrest at G0/G1-G2M, which was mediated by the down-regulation of expression of cell cycle regulatory proteins cyclin D1, CDK4 and cyclin B1, respectively.
31) reported cyclin B1 overexpression during the S phase fraction and in accordance with the present study although cyclin B1 expression was not observed during the S and G2/M phases in lymphocytes administered phytohemagglutinin to stimulate proliferation, its expression was high during all 3 phases in tumoral samples.
The study is entitled "Estrogen receptor beta causes a G2 cell cycle arrest by inhibiting CDK1 activity through the regulation of cyclin B1, GADD45A, and BTG2".
Paraffin-embedded specimens from inactive endometrium (n = 16), endometrial hyperplasia (n = 23) and endometrioid endometrial carcinoma (n = 39) were stained immunohistochemically for cyclin A, cyclin B1, cyclin D1, cyclin E, cdk2, p16, p21, p27, p53 and Ki67(MIB-1).
Imm unohistochemistry, an antibody reaction, is used to determine the expression of cyclin B1 within the cell.
The primary antibodies for phospho-ERKl/2, ERK1/2, phospho-Aktl and Aktl and Kipl/p27 were from Cell Signaling Technology (Beverly, MA, USA), beta-actin from Sigma, cyclin B1 from Merck Millipore, Billerica, MA, USA, and Cdc2 from Upstate Biotechnology, USA.
GEM also induced phosphorylation of JNK, activation of caspase-3 and accumulation of cyclin B1.