CCNB1

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CCNB1

A gene on chromosome 5q12 that encodes cyclin B1, which is essential for controlling the cell cycle at the G2/M transition. Cyclin B1 interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex, the maturation promoting factor, and interacts with RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase.
References in periodicals archive ?
The primary antibodies for phospho-ERKl/2, ERK1/2, phospho-Aktl and Aktl and Kipl/p27 were from Cell Signaling Technology (Beverly, MA, USA), beta-actin from Sigma, cyclin B1 from Merck Millipore, Billerica, MA, USA, and Cdc2 from Upstate Biotechnology, USA.
7 cells through cell cycle arrest at G0/G1-G2M, which was mediated by the down-regulation of expression of cell cycle regulatory proteins cyclin D1, CDK4 and cyclin B1, respectively.
31) reported cyclin B1 overexpression during the S phase fraction and in accordance with the present study although cyclin B1 expression was not observed during the S and G2/M phases in lymphocytes administered phytohemagglutinin to stimulate proliferation, its expression was high during all 3 phases in tumoral samples.
The study is entitled "Estrogen receptor beta causes a G2 cell cycle arrest by inhibiting CDK1 activity through the regulation of cyclin B1, GADD45A, and BTG2", in the journal Breast Cancer Research and Treatment.
Paraffin-embedded specimens from inactive endometrium (n = 16), endometrial hyperplasia (n = 23) and endometrioid endometrial carcinoma (n = 39) were stained immunohistochemically for cyclin A, cyclin B1, cyclin D1, cyclin E, cdk2, p16, p21, p27, p53 and Ki67(MIB-1).
Imm unohistochemistry, an antibody reaction, is used to determine the expression of cyclin B1 within the cell.
P21, a member of the CDKI family and cyclin B1 are the central players of G2/M phase transition (Vermeulen et al.
GEM also induced phosphorylation of JNK, activation of caspase-3 and accumulation of cyclin B1.
NIPA is part of a molecular complex that triggers degradation of cyclin B1 during the cell's "resting" phase, but allows it to accumulate as the cell gets ready to divide
DNA content and Cyclin B1 expression were measured using flow cytometry and a p53 functional human osteosarcoma cell line (U2-OS).