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cyanomethemoglobin

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cyanomethemoglobin
[sī′ənō′met·hē′məglō′bin]
Etymology: Gk, kyanos + meta, together with, haima, blood; L, globus, ball
a hemoglobin derivative formed during nitrite therapy for cyanide poisoning.


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A Nearly all current procedures for measuring hemoglobin involve red-blood-cell (RBC) lysis/dilution and formation of a cyanomethemoglobin compound, which is read by a spectrophotometer at 540 nm.
Following centrifugation at 800g for 5 min, the concentration of hemoglobin in the supernatant solution was determined using the cyanomethemoglobin method (Drabkin and Austin, 1935; International Committee for Standardization in Haematology, 1978).
The metHb will react with the cyanide to form cyanomethemoglobin that is bright red, but the sulfHb will remain chocolate brown.
 
 
 
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