Hemoglobin levels were determined by using the cyanomethemoglobin
method (HemoCue, Cypress, CA, USA).
The haemoglobin (Hb) content of blood was estimated by Drabkin cyanomethemoglobin
method (Drabkin, 1944).
Cyanide antidote kit additionally contains sodium thiosulfate which converts cyanomethemoglobin
to the less toxic thiocyanate (1).
1-3) Accurate measurement of the Hgb concentration in avian blood by the traditional cyanomethemoglobin technique requires lysis of erythrocytes followed by centrifugation to remove free nuclei.
Small, portable, point-of-care hemoglobinometers are commercially available that use a spectrophotometric double-wavelength azide-methemoglobin method to correct for sample turbidity resulting from lipid particles, cell stromata, and large proteins that tend to interfere with the cyanomethemoglobin method.
A Nearly all current procedures for measuring hemoglobin involve red-blood-cell (RBC) lysis/dilution and formation of a cyanomethemoglobin
compound, which is read by a spectrophotometer at 540 nm.
Following centrifugation at 800g for 5 min, the concentration of hemoglobin in the supernatant solution was determined using the cyanomethemoglobin method (Drabkin and Austin, 1935; International Committee for Standardization in Haematology, 1978).
A method of quantification has been devised in which hemoglobin or red blood cells leaking into the cutaneous tissue are eluted with saline solution and subsequently determined as cyanomethemoglobin (Drabkin and Austin, 1935; International Committee for Standardization in Haematology, 1978).
MetHb reacts with cyanide to form bright red cyanomethemoglobin
The metHb will react with the cyanide to form cyanomethemoglobin
that is bright red, but the sulfHb will remain chocolate brown.
3] resulting to cyanomethemoglobin
(CNmetHb) and reduced free cyanides in the blood.
525-A) is based on the colorimetric cyanomethemoglobin
method, and the Pr assay (Sigma cat.