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culture procedure

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culture procedure,
(in bacteriology) any of several techniques for growing colonies of microorganisms to identify a pathogen and to determine its sensitivity to various antibiotics. Usually a specimen is secured by using sterile technique and a small amount is placed into or on one or more culture media, because different organisms are nourished by different nutrients and grow best at different specific pH levels. The environment in which the media are held during observation is maintained at body temperature, and the ambient oxygen level is adjusted to achieve an aerobic or anaerobic state. All procedures are aseptic, and all equipment is sterile to prevent accidental contamination of the media. As colonies appear in or on the media, small amounts of each (there are often several) are spread onto other media to allow examination of a pure specimen of the microorganisms.


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Culture procedures were as follows: A sterile cotton swab was dipped into sterile water, and the swab was then used to wipe the entire area of either the mouthpiece, the handle inset, or the earpiece of a telephone.
Cell Culture Procedures for Assaying Plaque Forming Viruses, EPA Document Number EPA/600/4-84/013 (R-10).
The new Modified Butzler (ISO) freeze-dried Selective Supplement utilises amphoteracin B as an antifungal agent in place of cycloheximide, making it safer for laboratory staff to use in culture procedures.
 
 
 
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