culture procedure

culture procedure

(in bacteriology) any of several techniques for growing colonies of microorganisms to identify a pathogen and to determine its sensitivity to various antibiotics. Usually a specimen is secured by using sterile technique and a small amount is placed into or on one or more culture media, because different organisms are nourished by different nutrients and grow best at different specific pH levels. The environment in which the media are held during observation is maintained at body temperature, and the ambient oxygen level is adjusted to achieve an aerobic or anaerobic state. All procedures are aseptic, and all equipment is sterile to prevent accidental contamination of the media. As colonies appear in or on the media, small amounts of each (there are often several) are spread onto other media to allow examination of a pure specimen of the microorganisms.
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The tissue culture procedure is not only solving the problem of planting material, but also helping to increase production of bamboo saplings within a short span of time.
From 1993 to 1998, we correlated the results of Bartonella-positive cultures with the type of sample, the culture procedure, and polymerase chain reaction (PCR)-based genomic detection (11).
Our lab routinely performs five PCR tests (HSV, CMV, varicella-zoster virus [VZV], Epstein-Barr virus, and human herpes virus 6) on a single, 100-[Mu]l intra-ocular fluid specimen (a specimen volume barely sufficient for a single culture procedure.
Using a selective culture procedure with enrichment broth (bile esculin azide broth, Acumedia, LabFab, Ljusne, Sweden) containing vancomycin (8 [micro]g/ml) and aztreonam (60 [micro]g/ml), we isolated vancomycin-resistant E.
Using the Company's proprietary embryo culture procedure, approximately 200 Embryos will be harvested from swine in the United States and transported to one of the largest swine production facilities in the Philippines.
For example, before working up sputums on the routine microbiology bench", the trainee goes over the sputum culture procedure.
In the meantime, the manufacturer will use detailed information on our blood culture procedure to customize the program.
Reagent and media was the largest product market in 2014, owing to increasing number of cell culture procedures.
As such, the quality of the cultured cells and the efficiency of culture procedures are highly dependent on the proficiency and skill of each individual technician.
3% (296/4 037) of blood culture procedures, preferring clean but non-sterile gloves for the rest.
Scientists from Canada and elsewhere in the world detail such procedures as the neural differentiation of human embryonic stem cells, primary sensory and motor neuron cultures, oligodendrocyte progenitor cell culture, cerebellar slice cultures, live imaging of neural cell functions, and tissue culture procedures and tips.