Tenders are invited for Supply and installation of cryotome
for laboratory histopathologh and immunohistochemistry
Specimens for frozen section were frozen to -22 [degrees]C within 10 minutes in the Shandon Cryotome
SME Cryostat (Thermo Fisher Scientific, Inc.
Para el corte histologico de los encefalos se utilizo el criostato de congelacion Thermo Shandon Cryotome
The compact gross pathology room was equipped with a single Tissue-Tek VIP 5 tissue processor (Sakura, Torrance, California), a Cryotome
cryostat (Thermo Fisher Scientific, Waltham, Massachusetts) for potential intraoperative frozen sections, and other standard histology equipment, including a water bath and microtome to prepare glass slides from formalin-fixed, paraffin-embedded tissue (Figure 3).
Analyses were performed on 1-3 cm gastroduodenal segments, which were fixed in 10% (vol/vol) formalin, embedded in paraffin, and sectioned (6 [micro]m) using a Finesse microtome (Shandon Cryotome
, Fisher Scientific, Waltham, MA).
For maturation stage identification, they were cut into 3-[micro]m sections at -30[degrees]C with a cryostat (Thermo Shandon Cryotome
0620) and stained with Lee's methylene blue-basic fuchsin for microscopic examination (Gauthier-Clerc et al.
Sections 15 [[micro]meter] thick were made with a Hacker-Bright cryotome