Before assay, IGF-I was extracted with acid-ethanol cryoprecipitation
(Breier et al.
It is essential that fasting serum samples be collected at 37[degrees]C to avoid false positives due to cryoprecipitation
of lipoproteins or false negatives due to premature cryoprecipitation
of immunoglobulins, rheumatoid factor, and complement (3).
2) The processed serum is observed for cryoprecipitation
by daily inspection at 4[degrees]C for seven days.
Immune complex (IgM-IgG) cryoglobulinemia: the mechanism of cryoprecipitation
In her ISTH Congress presentation -- A Novel Automated Method of Cryoprecipitation
to Generate Autologous Fibrinogen for Fibrin Glue -- she concluded that the CryoSeal System offers an automated and rapid method for production of cryo from autologous source donations.
The design of the CryoSeal FS System is based upon two of the Company's recent technological breakthroughs enabling real time processing of blood products, including: (a) automated cryoprecipitation
technology which prepares fibrinogen-rich cryoprecipitate from plasma in as little as 51 minutes, as compared to two to three days for standard blood center methodology; and (b) thrombin activation technology which prepares activated human thrombin from a small aliquot of the same human plasma.
The IGF-I in plasma was extracted with acid-ethanol cryoprecipitation
and analyzed using specific radioimmunoassay kits consisting of anti-hIGF-I, [sup.
These cases remind us that IgM monoclonal proteins (M-proteins) have earned a reputation as saboteurs of many laboratory assays owing to their self-aggregation, aggregation of latex particles, binding to analytes and reagents, and cryoprecipitation
Others are made of a variety of materials designed to selectively retain specific plasma components by cryoprecipitation
(removal of cryoglobulins) or affinity adsorption (such as removal of IgG-class antibodies by adsorption to Staphylococcus protein A).
Although most authors report performing cryoprecipitation
only at 4 [degrees]C, some authors find that cryoprecipitation
at different temperatures has merit (10).
Cryoglobulins that precipitate significantly at 20 to 30 C and as high as 36 C have been described(2) and may be associated with more serious symptomatology than other cryoglobulins with a lower cryoprecipitation
point, present in significantly higher concentrations.
technology prepares fibrinogen-rich cryoprecipitate from plasma in as little as 40 minutes as compared to two to three days for standard blood center methodology, while our patented thrombin activation technology prepares activated human thrombin from a small volume of the same human plasma.