1, they indicate that the cryocrit should be performed on "washed precipitate," but in the Discussion they state that the cryocrit is the "(precipitate volume)/ (total volume of serum sample).
From a theoretical standpoint, the use of the cryocrit is rather dubious because it assumes that different cryoproteins pack and sediment with equal volumes.
Other investigators have reported an apparent inverse relationship between the cryocrit and vasculitis (2).
Sixty percent of patients who received IFN-[alpha] had undetectable levels of HCV RNA, and in all of these patients signs and symptoms of cryoglobulinemia disappeared with significant improvement in serum cryocrit levels and IgM RF.
36) These patients with palpable purpura had significantly higher serum cryocrit levels than patients without purpura.
In the setting of a chronic, untreated HCV infection and evidence of nephrotic syndrome, a cryocrit was requested to investigate the possibility of membranoproliferative glomerulonephritis secondary to cryoglobulinemia.
A cryocrit of 5% was reported for 2 blood samples drawn on different days after storage of serum at 4[degrees]C for 10 days.
Some laboratories determine cryocrit, and others quantify total protein content.
Of the 127 laboratories included for the postidentification phase, 50 (39%) reported the cryocrit, the total protein content, and/or the immunoglobulin concentration in the cryoprecipitate (quantitative); 48 (38%) answered negative/ positive only; and 29 (23%) provided additional information without reporting the cryoglobulin concentration in the cryoprecipitate (nonquantitative).
a) Patient Cryoglobulin Cryocrit
, Peak at Peak at no.
Patient Disease Cryocrit
, % 1 Lymphoplasmacytoid immunocytoma 7 2 Waldenstr6m macroglobulinemia 15 (immunocytoma) 3 Centroblastic lymphoma 4 4 Waldenstrom macroglobulinemia 40 (immunocytoma) (Gelatinous appearance) CD4000 CD4000 optical impedance Patient count count 1 355 356 2 619 578 3 94 66 4 476 572 CD4000 Technicon H*3 immunoplatelet platelet Patient (CD61) count count 1 42 445 2 226 819 3 21 23 4 329 422 Hemacytometer (chamber) Patient count 1 38 2 289 3 22 4 318
After blood is collected at 37[degrees]C and serum separated, 5 mL of the serum is allowed to stand in a cryocrit
tube at 4[degrees]C for 2-7 days, and formation of the precipitate is confirmed visually.