The transmission electron microscope Titan Krios for cryo-EM
and cryo-electron tomography with energy filters including direct electron detectors and newly developed phase plate represents the best technical solution available for the elucidation of molecular structures by means of electron microscopy.
Having just recently broken the 3 Ea (angstrom) barrier with the use of the Gatan K2 Summit direct detection camera, a new structure shows that there is no longer a resolution gap between x-ray crystallography and cryo-EM.
2 Ea resolution cryo-EM structure of [eth]-galactosidase in complex with a cell-permeant inhibitor, 10.
is likely to reveal much more about bacterial "bones," features that were invisible with traditional EM.
The Titan Krios will be located in the state-of-the-art NUS Cryo-EM
facility, led by Prof.
High-resolution cryo-EM can, therefore, provide information about protein dynamics, which is key to understanding the functions of many macromolecular complexes.
This advantage of Cryo-EM is of major interest to the pharmaceutical industry.
The detector will provide MRC-NIMR with state-of-the-art digital acquisition for high-resolution cryo-EM
for a wide variety of research applications, including high resolution single particle analysis and cryotomography of cellular specimens.
Employing an integrated structural biology approach using single-particle cryo-EM
and X-ray crystallography, this proposal aims to elucidate the structural details by which the autophagosomal recognition machinery identifies and assembles its cargo for targeting to autophagosomes.
Total quantity or scope: Lots A and B describe a combined (high-voltage and energy-filtered) cryo-EM
instrument for use in a wide range of different research projects from single particle EM to dual-axis cryo-tomography, using the best state-of-the-art technology.
High resolution cryo-EM
allows the study of all these structures and interactions at the 2D and subsequently 3D level, provided that the natural state of these structures is well preserved by cryo fixation," added Fastenau.
We combined the X-ray structure of ISW1a(ATPase) bound to DNA with cryo-EM
structures of the factor bound to two different nucleosomes to build a model showing how this remodeler uses a dinucleosome, not a mononucleosome, as its substrate.