The other bacteria which produce creatinase are as follows: Pseudomonas (2,24), Bacillus (25), Flavobacterium (1), Micrococcus (26), Alcaligenes, Clostridium (21), Athrobacter (28), and Paracoccus (9).
Because creatinase is an enzyme that is used for clinical application, its activity is an important property.
The aim of this study was to investigate if ATCC-12633 creatinase has better activity than commonly used commercially creatinases.
putida ATCC12633 was used as the source of creatinase gene (Persian Type Culture Collection (IRO-ST PTCC, Iran)).
Creatinine: This enzymatic method is based on the conversion of creatinine with the aid of creatininase, creatinase, and sarcosine oxidase to glycine, formaldehyde, and hydrogen peroxide.
Creatine of the sample is destroyed by creatinase, SOD, and catalase during incubation in R1.
Contract awarded for Ern laboratory - march biochemicals (eci psa, phenytoin, amylase, ck creatinase
, ckmb, ggt, lactate, lipase, protein csf, transferrin, eci beta, etc
Ern laboratory - march biochemicals (eci psa, phenytoin, amylase, ck creatinase, ckmb, ggt, lactate, lipase, protein csf, transferrin, eci beta, etc
Creatine is then converted to sarcosine and urea via creatinase
Urine creatinine concentrations were measured using Creatinine Plus Assay, which is a coupled enzymatic reaction using creatininase, creatinase
, and sarcosine oxidase (Roche Diagnostic, Indianapolis, Ind).
This method is based on the same reaction sequence as the Creatinine-PAP (phenol aminophenazone) method (29) consisting of 4 consecutive enzymatic steps via creatininase, creatinase
, sarcosine oxidase, and peroxidase, but using a different chromophore (2,4,6-triiodo-3-hydroxybenzoic acid) and a modified detergent composition.
The enzymatic method is based on the determination of sarcosine after conversion of creatinine with creatininase, creatinase
, and sarcosine oxidase.
One method was based on coupling of enzymatic bioassays using creatininase, creatinase
, and sarcosine oxidase and electrophoresic separation and amperometric detection of reaction products (46).