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creatinase

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cre·a·ti·nase (kr-t-ns, -nz)
n.
An enzyme that catalyzes the hydrolysis of creatine to sarcosine and urea.

creatinase
[krē·at′i·nās]
Etymology: Gk, kreas, flesh
an enzyme that catalyzes the conversion of creatine to sarcosine and urea.

creatinase [kre-at´ĭ-nās]
an enzyme that catalyzes the decomposition of creatine into urea and ammonia.

creatinase
an enzyme that catalyzes the decomposition of creatine into urea and ammonia.


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This method is based on the same reaction sequence as the Creatinine-PAP (phenol aminophenazone) method (29) consisting of 4 consecutive enzymatic steps via creatininase, creatinase, sarcosine oxidase, and peroxidase, but using a different chromophore (2,4,6-triiodo-3-hydroxybenzoic acid) and a modified detergent composition.
The enzymatic method is based on the determination of sarcosine after conversion of creatinine with creatininase, creatinase, and sarcosine oxidase.
One method was based on coupling of enzymatic bioassays using creatininase, creatinase, and sarcosine oxidase and electrophoresic separation and amperometric detection of reaction products (46).
 
 
 
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