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The coverslips were rigorously cleaned previously by boiling in piranha solution (70% reagent grade sulfuric acid, 30% of 30% hydrogen peroxide) for 1 h at 90°C, followed by thorough rinses with deionized water, and then air-dried. After hybridization, rubber cement and coverslips were removed. 9% pure atrial cardiomyocytes in various formats, including frozen cryovials, pre-seeded 96-well plates, and pre-seeded coverslips. |
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