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The researchers patterned four platinum electrodes onto a glass coverslip, 'like a printed circuit board," Lindau says. After glycerol-PBS mounting medium and coverslip were applied, the slides were read at a magnification of 400x with an epifluorescence UV microscope. After 20 min DNA staining in ToPro-3-iodide (Molecular Probes; 10 [micro]M in PBS, prepared from 1 mM stock solution in dimethyl sulfoxide) and 5 min of washing in PBS, cytoslides were mounted with equilibration solution and antifade reagent (SlowFade Light Antifade Kit; Molecular Probes) and sealed with coverslips. |
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