counterstain

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counterstain

 [kown´ter-stān]
a stain applied to render the effects of another stain more discernible.

count·er·stain

(kown'ter-stān),
A second stain of different color having affinity for tissues, cells, or parts of cells other than those taking the primary stain used to render more distinct the parts taking the first stain.

counterstain

/coun·ter·stain/ (-stān) a stain applied to render the effects of another stain more discernible.

counterstain

(koun′tər-stān′)
n.
A stain of a contrasting color used to color the components in a microscopic specimen that are not made visible by the principal stain.
tr.v. counter·stained, counter·staining, counter·stains
To color (a microscopic specimen) with such a stain.

counterstain

a second stain added to a previously stained tissue sample to make cellular details more distinct.

count·er·stain

(kown'tĕr-stān)
A second stain of a different color, having affinity for tissues, cells, or parts of cells other than those taking the primary stain, used to render more distinct the parts taking the first stain.
Synonym(s): contrase stain.

counterstain

a stain applied to render the effects of another stain more discernible.
References in periodicals archive ?
Overdecolorization is still possible once the counterstaining step is reached.
A comparison of melanin bleaching and azure blue counterstaining in the immunohistochemical diagnosis of malignant melanoma.
We visualized the presence of antigen on the cell surfaces using a Vectastain ABC kit (Vector Laboratories) with diaminobenzidine tetrahydrochloride (DAB) as a substrate followed by counterstaining with hematoxylin.
The reaction was visualized by incubating the sections with diaminobenzidine (Sigma, St Louis, Mo) and counterstaining with hematoxylin.
Immunohistochemical testing was performed with rabbit anti-B, quintana antibody (1:1,000) and hematoxylin counterstaining as described previously (8).
The tissue sections were treated with anti-Histag antibody with appropriate secondary antibody with the counterstaining of hematoxylin.
The staining protocol included epitope retrieval, sequential use of peroxidase-blocking reagent and primary antibody, addition of visualization reagent, application of substrate chromogen solution (diaminobenzidine), counterstaining with hematoxylin, and mounting in a nonaqueous medium.
Diaminobenzidine (Vector) was then applied for 5 minutes, followed by washing and counterstaining with Harris hematoxylin for 1 minute.
The receptor-negative cells are blue because of counterstaining of the tissue with hematoxylin-eosin.
The reaction product was then visualized by applying diaminobenzidine (DAB, Vector Laboratories) working solution for 5 minutes, followed by washing in tap water and counterstaining with Harris hematoxylin for 2 minutes.