counterstain


Also found in: Dictionary, Thesaurus, Encyclopedia, Wikipedia.

counterstain

 [kown´ter-stān]
a stain applied to render the effects of another stain more discernible.

count·er·stain

(kown'ter-stān),
A second stain of different color having affinity for tissues, cells, or parts of cells other than those taking the primary stain used to render more distinct the parts taking the first stain.

counterstain

/coun·ter·stain/ (-stān) a stain applied to render the effects of another stain more discernible.

counterstain

(koun′tər-stān′)
n.
A stain of a contrasting color used to color the components in a microscopic specimen that are not made visible by the principal stain.
tr.v. counter·stained, counter·staining, counter·stains
To color (a microscopic specimen) with such a stain.

counterstain

a second stain added to a previously stained tissue sample to make cellular details more distinct.

count·er·stain

(kown'tĕr-stān)
A second stain of a different color, having affinity for tissues, cells, or parts of cells other than those taking the primary stain, used to render more distinct the parts taking the first stain.
Synonym(s): contrase stain.

counterstain

a stain applied to render the effects of another stain more discernible.
References in periodicals archive ?
All slides were washed with tap water; hematoxylin counterstain was applied, followed by dehydration with absolute alcohol, clearing with xylene, and coverslipping with a permanent mounting media.
Both can be visualized well against the blue-black nigrosin counterstain (see Figure 3).
Gaithersburg, MD, USA) was then applied to the slides, which were incubated and washed as before; Eriochrome Black T counterstain was added to the middle wash.
Gonad samples were prepared for histological examination with a modification of the periodic acid Schiff's (PAS) stain for glycol-methacrylate sections and with Weigert's iron-hematoxylin as a nuclear stain and metanil yellow as a counterstain (Quintero-Hunter et al.
With conventional light microscopy, a practical limit of about 3 to 4 colors can be usefully resolved, including any needed counterstain (eg, hematoxylin).
Tissues were embedded in paraffin, sectioned at 5[micro]m, and stained with hematoxylin followed by eosin counterstain.
Tissues were embedded in paraffin, sectioned at 5 [micro]m and stained with Harris' hematoxylin followed by eosin counterstain (Presnell & Schreibman 1997).
Gaithersburg, MD) was then applied to the slides, which were incubated and washed as before, except that eriochrome black Y counterstain was added to the middle wash.
For slides that were used for image quantification, no counterstain was applied to simplify image colorimetric quantification.
A single series of sequential, histological sections (6 [micro]m thick) (2-8: mode=3[ovaries], mode=4[testes]) was cut and stained with Harris's hematoxylin, followed by eosin counterstain (Hunter and Macewicz, 1985).