counterimmunoelectrophoresis


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counterimmunoelectrophoresis

 (CIE) [kown″ter-im″u-no-e-lek″tro-fo-re´sis]
a laboratory technique in which an electric current is used to accelerate the migration of antibody and antigen through a buffered diffusion medium. Antigens in a gel medium in which the pH is controlled are strongly negatively charged and will migrate rapidly across the electric field toward the anode. The antibody in such a medium is less negatively charged and will migrate in an opposite or “counter” direction toward the cathode. If the antigen and antibody are specific for each other, they combine and form a distinct precipitin line.

The technique of CIE was first applied clinically in 1970 to detect hepatitis B antigen. With modification and refinement it is becoming increasingly useful as a means of detecting antigens or antibodies specific for a variety of infectious diseases. It can be especially valuable as an aid to accurate diagnosis of clinical bacterial infections and the selection of specific therapeutic agents for control of infections once the causative organisms are identified.

count·er·im·mu·no·e·lec·tro·pho·re·sis (CIE),

(kown'ter-im'yū-nō-ĕ-lek'trō-fōr-ē'sis),
A modification of immunoelectrophoresis in which antigen (for example, serum containing hepatitis B virus) is placed in wells cut in the sheet of agar gel toward the cathode and antiserum is placed in wells toward the anode; antigen and antibody, moving in opposite directions, form precipitates in the area between the cells where they meet in concentrations of optimal proportions.

counterimmunoelectrophoresis

/coun·ter·im·mu·no·elec·tro·pho·re·sis/ (-im″u-no-e-lek″tro-for-e´sis) immunoelectrophoresis in which the antigen and antibody migrate in opposite directions.

count·er·im·mu·no·e·lec·tro·pho·re·sis

(kown'tĕr-im'yū-nō-ĕ-lek'trō-fŏr-ē'sis)
Immunoelectrophoresis in which antigen is placed in wells cut in the sheet of agar gel toward the cathode, and antiserum is placed in wells toward the anode; antigen and antibody, moving in opposite directions, form precipitates in the area between the cells where they meet in concentrations of optimal proportions.
References in periodicals archive ?
Screening of antibodies to extractable nuclear antigens was done by counterimmunoelectrophoresis (9) and identification by dot-blot analysis (Biomedical Diagnostics) (12).
Human rotavirus detection by counterimmunoelectrophoresis versus enzyme immunoassay and electron microscopy after direct ultracentrifugation.
Traditionally, anti-RNP antibodies have been detected by techniques such as passive hemagglutination, immunodiffusion, counterimmunoelectrophoresis, and ELISA using purified antigen.
md 372 28 3-Methyl-6-isopropylcyclohexanone, OED menthone 29 counterimmunoelectrophoretics, from counterimmunoelectrophoresis, Dorland 32 Daughters of the American Revolution, OED daughter 2b 373 25 to put a person upon his honour, OED honour 9d 27 erythrothrombomonoblastosis, allegedly Stedman 28 Fou-hsin-meng-ku-tsu-tzu-chih-hsien, China, 42[degrees]03, 121[degrees]44 30 2-methyl-3-difarnesyl-1,4-naphthoquinone, OED phylloquinone, 1953q 374 27 ventriculocisternostomizing, from ventriculocisternostomy, books.
4,94,111,112) To distinguish recent from remote infection, counterimmunoelectrophoresis and gel diffusion are more appropriate because they remain positive for only 6 to 12 months after the onset of infection.