cotransfection

cotransfection

/co·trans·fec·tion/ (ko″trans-fek´shun) simultaneous transfection with two separate, unrelated nucleic acid molecules, one of which may contain a gene that is easily assayed and acts as a marker.
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50) demonstrated that the inhibition of NF-[kappa]B by cotransfection of a superrepressor form of I[kappa]B[alpha] caused a dramatic loss of cell viability.
The responsiveness of the Ishikawa(-) cells could be restored by cotransfection with either an hER[alpha] or hER[beta] expression plasmid, although, overexpression with hER[beta] consistently produced a lower activity profile.
Indeed, cotransfection experiments with two mutant GTPCH alleles, identified in DRD patients, demonstrated that mutant proteins inhibit the activity of the wild-type enzyme.
In cardiomyocytes transfected with pGL2(-642) GDF15, cotransfection of the FSTL1 expression plasmid resulted in a 5.
Our data showed that cotransfection of Cdx2 with Jun and Ets2 into JEG-3 cells resulted in the highest ovine IFNT gene transcription, which is in agreement with our previous study (Imakawa et al.
Similarly, GFP-expressing vector constructs based on HIV-1 (HIV-CLCG), SIV (VCGDBH) and MLV (pL-EGFP-Nl) were used to generate the respective vector particles by cotransfection with pHIT-G and the respective gag-pol expression plasmids ([Hgp.
For example, upon cotransfection of HEK293 cells with both the BK channel main a-subunit and an auxiliary [beta]1-subunit, the resulting BK channel is composed of [alpha]-[beta]1 complexes.
Retroviral particles pseudotyped with SARS-CoV S were made by cotransfection of an S-expressing plasmid, pCAGGS-S, with plasmids encoding MLV or HIV gag-pol and GFP vector genome in 293T cells.
Finally, a stable cell line will be generated from cotransfection of this QC expression vector and a selection vector, pCoHYGRO.
Cotransfection of Ahr siRNA and Email plasmid or Email siRNA and Ahr plasmid does not rescue Ppara down-regulation induced by silencing of Ahr or Email [see Supplemental Material, Figure 3 (http://dx.
Furthermore, both plasmid vector transfection and cotransfection were carried out.