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cotransfection

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cotransfection /co·trans·fec·tion/ (ko″trans-fek´shun) simultaneous transfection with two separate, unrelated nucleic acid molecules, one of which may contain a gene that is easily assayed and acts as a marker.


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Cotransfection with a plasmid carrying a neo expression cassette and selection with G418 enabled us to isolate individual clones of hPNK-depleted A549 cells.
Results Production of MLV S Pseudotypes Retroviral particles pseudotyped with SARS-CoV S were made by cotransfection of an S-expressing plasmid, pCAGGS-S, with plasmids encoding MLV or HIV gag-pol and GFP vector genome in 293T cells.
 
 
 
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