The Candida isolates obtained were further identified by conventional methods such as gram stain (direct microscopy), germ tube test, Sabouraud dextrose broth, microscopic morphology on cornmeal agar
, and sugar assimilation tests.
Speciation of Candida using chromogenic and cornmeal agar
with determination of fluconazole sensitivity.
Cornmeal agar was purchased from Becton, Dickinson and Co, Sparks, MD, USA.
API tests and cornmeal agar were found to be best conventional methods for the identification of all Candida species but however in our present settings of resources constrained laboratories it cannot be used routinely.
albicansa single colony of Candida was picked from the a pure culture medium and inoculated on a plate of Cornmeal agar
containing 1%Tween 80 and rice agar containing 0.
Carbohydrate assimilation tests (ID32C system) were the second method of identification used instead of cornmeal agar
culture because the ID32C system has the capability to identify many Candida species (in excess of 30) and several non-Candida yeasts as well.
1) The organism grows quickly on Sabouraud dextrose agar, blood agar, or cornmeal agar
as a septate, branched hyphal mold which may be difficult to morphologically discern from Aspergillus spp.
The most common media are Sabouraud, Malt Agar, Cornmeal agar
, Potato-dextrose agar, and Staib agar.
Cleistothecia were observed after 2 weeks of incubation on potato dextrose agar and cornmeal agar
(Figure 1, C).
6] Germ-tube testing and morphology on cornmeal agar
were also performed to enable the accurate identification of Candida spp.
For fungal culture, specimen was inoculated on Sabouraud's agar and cornmeal agar
at 25 degree celsius as well as at 37 degree celsius.
The organism was subcultured on Cornmeal Agar
and brown-black cleistothecia containing numerous ascospores were observed following 14 days of incubation.