METHODS AND RESULTS: By screening the affinity of RDX for a number of neurotransmitter receptors, we found that RDX binds exclusively to the picrotoxin convulsant site of the [gamma]-aminobutyric acid type A ([GABA.
A] receptor convulsant site is the primary mechanism of seizure induction by RDX and that reduction of GABAergic inhibitory transmission in the amygdala is involved in the generation of RDX-induced seizures.
Here, we present evidence that RDX binds at the convulsant site of the [gamma]-aminobutyric acid type A ([GABA.
Having found that RDX binds to the convulsant site of the [GABA.
Key words: Nantenine, Aporphine, Alkaloid, Anticonvulsant activity, Convulsant activity
The convulsant activity previously observed at high doses was also investigated.
Fisher's exact test was performed to determine significance of other anticonvulsant and convulsant properties results.
Nantenine showed a dose-dependent convulsant activity at doses ranging from 75 to 105 mg/kg.
At 7 days postfertilization (dpf), after having undergone completion of brain formation at 5 dpf, zebrafish exhibit behavioral, electro-graphic, and molecular changes to chemical convulsants that would be expected from a rodent seizure model.
In addition to enhancing induced seizure behavior, p,p '-DDE also promotes a unique and readily observed head-shake behavior in response to the two chemical convulsants (Tiedeken and Ramsdell 2009).
After brain maturation (7 days postfertilization), fish were exposed to a chemical convulsant, either pentylenetetrazole or domoic acid; resulting seizure behavior was then monitored and analyzed for changes, using cameras and behavioral tracking software.
Additionally, we administered this complex formulation by egg microinjection rather than bath exposure to assure consistent internal composition at the time of exposure to the chemical convulsant.