GGH

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GGH

A gene on chromosome 8q12.3 that encodes gamma-glutamyl hydrolase, which progressively removes gamma-glutamyl residues from pteroyl poly-gamma-glutamate to yield pteroyl-alpha-glutamate (folic acid) and free glutamate. GGH may play a key role in ensuring the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates.
References in periodicals archive ?
Samples were removed every three hours, heat-treated and treated with conjugase before the microbiological assay was performed.
2, which favor plasma conjugase activity, and a large cohort of cells remained intact.
The extract (50 [micro]L) was incubated for 2 h at 37[degrees]C with chick pancreas conjugase (10 [micro]L), prepared as described by Mims and Laskowski (15), to convert the folates to their monoglutamyl forms.
Preliminary studies with standard reduced polyglutamyl and monoglutamyl folate, using HPLC for folate analysis, indicate that there is a loss of folate activity during the incubation with conjugase (data not shown).
n]) with plasma folate conjugase ([gamma]-glu-x carboxypeptidase; EC 3.
That total lysis of all red blood cells will liberate erythrocyte folate completely to unfettered access by plasma folate conjugase and consequently ensure complete RCF deconjugation to the monoglutamate form before analysis by either MA or RFBP assay;
Knowing that 10-fold dilution of whole blood will produce total lysis is not synonymous with the assumption that there is complete liberation of RCF to unfettered access by plasma folate conjugase.
The optimal deconjugation of folyl-polyglutamates with folate conjugase enzyme proceeds as a function of buffer, pH, amount of enzyme, temperature, and reaction time (18).