confocal microscopy

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Related to confocal microscopy: fluorescence microscopy

confocal microscopy

Microscopy that permits high-resolution analysis of serial optical sections (microscopic tomograms) into the depths of tissues or cells.
See also: microscopy


examination with a microscope.

confocal microscopy
a technique for obtaining high resolution images and 3-D reconstructions of biological specimens; a laser light beam is expanded to make optimal use of the optics in the objective lens and is turned into a scanning beam via an x-y deflection mechanism and is focused to a small spot by the objective lens onto a fluorescent specimen. The mixture of reflected light and emitted fluorescent light is captured by the same objective and after conversion into a static beam by the x-y scanner device is focused onto a photodetector (photomultiplier) via a dichroic mirror (beam splitter) to create the final image. Called also laser scanning microscopy; confocal scanning laser microscopy.
immunoelectron microscopy
the mixing of antibody with an antigen such as a virus on a specimen grid so as to increase the probability of visualizing a virus and to identify (type) the kind of virus present in the specimen, or antibody may be conjugated with gold and used to visualize and determine the location of specific antigenic determinants on a specimen.
References in periodicals archive ?
Guitera P Pellacani G, Crotty KA, et al: The impact of in vivo reflectance confocal microscopy on the diagnostic accuracy of lentigo maligna and equivocal pigmented and nonpigmented macules of the face.
When conjugated to antibodies that bind specifically to proteins present on the surface of cancer cells but not on normal cells, thousands or millions of nanoparticles will attach to a single cancerous cell, and this high density causes the cell membrane to appear many times brighter than normal cells when viewed using confocal microscopy.
In this study, we aim to contribute to the elucidation of the arrangement of the placental microvascular bed by 3-D renderings of villous vessels, using confocal microscopy and tools of computer-based visualization.
We have shown, by use of confocal microscopy and transmission electron microscopy, internalization of Eu[P0.
Further analysis of individual cells with confocal microscopy "showed that hundreds of marrow-derived cells in brain sections expressed gene products typical of neurons," they reported.
Excitation source for high contrast, deep imaging in confocal microscopy and flow cytometry
1 confocal microscopy system to be assigned to soc 2 department of experimental oncology translational research cro of aviano.
It combines two separate technologies -- confocal microscopy and fluorescence lifetime imaging -- to noninvasively evaluate both the structural changes of tissue as well as molecular changes that take place on a cellular and tissue level.
Confocal microscopy was first described in the 1950s by Minsky (5) and is now a relatively mature technology with many commercial implementations.
His new computer model takes slices of laser confocal microscopy images of cells and reconstructs a whole, virtual version of an individual cell, allowing researchers to evaluate how that cell will respond to different mechanical stimuli.
Other topics include low-shrinkage composites, simultaneous fluorescence and reflection confocal microscopy of living cell interactions, the fracture resistance of non-metal posts, and an assessment of genetic damage and cellular death.
The confocal microscopy segment is the smallest of the surface science techniques (IBO's surface science category excludes confocal systems that are dedicated units for microarray scanning and other purposes).