However, deletions extending into regions adjacent to the transgene concatemer
are not detected, as two intact cos sites are required for the packaging of a single [lambda] vector.
sequences were analyzed by using SAGE software (version 4.
Briefly, DNA fragments were repaired to blunt-ended DNA by fill-in and exonuclease after A-tailing was conducted to prevent the formation of adapters, dimers, and concatemers
Lambda DNA concatemers
(New England BioLabs) were used as molecular size markers.
In addition, the PCR products were also analyzed by agarose gel electrophoresis to test for concatemers
and the correct product size of 425 bp.
of lambda DNA were used as molecular size markers (BioRad, Veenendaal, the Netherlands).