Fusing two single stranded DNA molecules by complementary base pairing
is also called annealing.
The students took on character roles of DNA and RNA while acting out complementary base pairing
, splicing of introns and joining of exons, matching of mRNA codons to the corresponding amino acids, and assembly of amino acids on a growing polypeptide chain.
However, Taq was eventually found to be susceptible to errors in complementary base pairing
during primer extension (see section on PCR protocol); and although the Taq polymerase is still used, many investigators currently prefer to use the DNA polymeruse cloned from Pyrococcus furiosus (Pfu), an organism that grows optimally in geothermal marine sediments at 100 [degrees] C.
Next, the temperature is lowered to about 60 C, causing the primers to bind to the separated strands by complementary base pairing