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competitive binding assay

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com·pet·i·tive binding assay (km-pt-tv)
n.
An assay in which a biologically specific binding agent competes for radioactively labeled or unlabeled compounds, used especially to measure the concentration of hormone receptors in a sample by introducing a radioactively labeled hormone.


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Competitive binding assays indicated that 17[beta]-estradiol, bisphenol A, and diethylstilbestrol shared a common membrane-binding site.
From the concept of this analytical approach came the competitive binding assay, an innovation that helped place almost all biologically important substances-including hormones, vitamins, polypeptides, pressor amines, and pharmaceutical agents-within range of analysis and greatly increased the scope and range of clinical laboratory testing.
 
 
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