competitive binding assay

com·pet·i·tive bind·ing as·say

general term for an assay in which a substance competes for labeled versus unlabeled ligand; following separation of free and bound ligand, the concentration of unlabeled ligand is inversely proportional to the amount of labeled bound ligand. Values are compared with known standards.
See also: enzyme-linked immunosorbent assay, radioreceptor assay, immunoassay, enzyme-multiplied immunoassay technique, radioimmunoassay.

com·pet·i·tive bind·ing as·say

(kŏm-pet'i-tive bīnd'ing as'ā)
An assay in which a binder competes for labeled versus unlabeled ligand; following separation of free and bound ligand, the ligand is quantitated by relating bound and unbound ratios to known standards.
See also: enzyme-linked immunosorbent assay, immunoassay, enzyme-multiplied immunoassay technique, radioimmunoassay
References in periodicals archive ?
Binding to PPAR-a, PPAR-p/5 and PPAR-y was assessed using a competitive binding assay, with the study finding LBE had affinity for and activated the three PPARs at 0.
A detailed description of the PPAR[gamma] binding assay is provided in Supplemental Material, "PPAR[gamma] Competitive Binding Assay and Quality Assurance/Quality Control.
Following the saturation assay, a set of competitive binding assay were performed in membrane expressing n opioid receptors, 5 opioid receptors and k opioid receptors to determine the binding affinity of mitragynine, using the tracer [[sup.
The PathHunter[TM] technology provides competitive binding assay pharmacology in a cell based assay platform.
Following the saturation assay, a set of competitive binding assay were performed in membrane expressing [mu] opioid receptors, [delta] opioid receptors and [kappa] opioid receptors to determine the binding affinity of mitragynine, using the tracer [3H]diphrenorphin and [[sup.
I was convinced of the power of this new approach to evaporation control when our scientists used the technology described in our new patent to reduce the volume of an aqueous competitive binding assay from 350 uL to 100 pL, more than a million times smaller.
In collaboration with Novalon Pharmaceutical Corporation, Cubist will also be presenting data on the identification of potent inhibitor molecules discovered in a new high throughput screening competitive binding assay that overcomes obstacles inherent in the screening of novel targets.
12] content of the sequestered holo-TC is released under reducing and alkaline conditions, converted to the stable cyano form with potassium cyanide, and quantified in a competitive binding assay with [[sup.
In the competitive binding assay reagent sets for total VEGF, ELISA plates usually are coated with goat antirabbit antibodies for capture of polyclonal rabbit antihuman VEGF antibody.
The specimen showed much less interference in a competitive binding assay, suggesting that the interference was produced by weak antibodies that did not compete well with a specific antigen, but could bridge the capture and detection antibodies together in a two-site assay.
had special characteristics for the competitive binding assay kit (Wako, Osaka, Japan).

Full browser ?