University of Alaska, Fairbanks: A Vertical Comet Assay
for Measuring DNA Damage to Radiation
In the comet assay
, cells were processed in alkali conditions and underwent submarine electrophoresis (Faccioni et al.
In addition to our patented human cell GADD45a reporter assays for early screening (GreenScreen HC and BlueScreen(TM) HC), we now offer an expanded portfolio of screening assays, such as Ames MPF(TM) and Microflow(TM) MNT, as well as a range of mechanistic follow-up assays including in vivo MNT, H2AX by flow cytometry, Apoptosis induction by Annexin-V binding and caspase 3/7 activity, and the Comet assay
The analyzer shall be equipped with an environmental control life support cultured cells during the measurements (5% CO2, at 370C) and optics and software for evaluation of multiple cell biological processes, including in particular: the cell cycle, transfer of test compounds through membranes and transport intracellular cytotoxicity and genotoxicity assay based on the formation of micronuclei and comet assay
who also observed a positive correlation between cumulus cells apoptosis and fertilization rate after ICSI, in this case using the Comet assay
The extent of apoptosis was assessed by comet assay
The researchers used two common in vitro assays, the Comet assay
and the long amplicon-qPCR assay, to test for genotoxicity and found that thirdhand smoke can cause both DNA strand breaks and oxidative DNA damage, which can lead to gene mutation.
To determine the level of damage of the DNA of human lymphocytes, the technique single cell gel electrophoresis (SCGE) or comet assay
was utilized according to the protocol described by Singh and co-authors (1988) with slight modifications.
The alkaline Comet Assay
or single cell gel electrophoresis (SCGE) assay is a rapid, sensitive and relatively simple method for DNA double--and single-strand breaks, alkali-labile sites and delayed repair site detection, in eukaryotic individual cells [13,14,15].
Neat semen was analysed for ROS levels by chemiluminescence (2) and DNA damages was assessed by comet assay
The extent of DNA damage was evaluated by the frequency of micronuclei in cultured peripheral blood lymphocytes and by comet assay
6] Buschini A, Martino A, Gustavino B, Monfrinotti M, Poli P, Rossi C, Santoro A, Dorr AM and Rizzoni M (2004) Comet assay
and micronucleus test in circulating erythrocytes of Cyprinus carpio specimens exposed in situ to lake waters treated with disinfectants for potabilization.