comet assay


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comet assay

A rapid method for detecting DNA damage in individual cells, which is simple and faster than conventional genotoxicity techniques—e.g., cytogenetics. The name derives from use of the tail moment of the electropherogram for evaluating DNA damage, which is defined as the product of the tail length and the fraction of the total DNA content in the tail of the electropherogram
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2008), we used the alkaline comet assay in this study to determine the various sperm DNA damages caused by PAHs, that is, base-free sites, single- and double-stranded DNA damages (Sakkas and Alvarez 2010).
Alkaline single cell gel electrophoresis (SCGE) assay or Comet assay was used to evaluate sperm DNA damage in infertile patients.
The oxidative status of trout blood tissues and the comet assay was used to measure DNA strand breaks in blood erythrocytes.
Here, we have carried out studies including anti-proliferation against human leukaemia cell lines, K562, THP-1 CCRF-CEM and CCRF-CEM/C1 (a camptothecin resistant cell line), topoisomerase relaxation and decatenation assays, measurement of DNA strand breaks using the comet assay, DNA-intercalating and footprinting assays, and cell cycle analysis.
Previous work in our laboratory showed that this freezing method produced comet assay results that were highly correlated with results from fresh, unfrozen samples (Duty et al.
In the present study, the effect of exposure to ascorbic acid (vitamin C) after gamma-ray-induced chromosomal damage in cultured human lymphocytes was examined to explore the mechanism by which this antioxidant vitamin protects irradiated cells Non-irradiated lymphocytes were exposed to increasing concentrations of ascorbic acid (1-100 micro g/ml) and DNA damage was estimated using chromosomal aberration analysis and the comet assay.
5 ml of blood was collected by retroorbital sinus for comet assay and later the mice were sacrificed to aspirate the femoral bone marrow for micronueleus test.
Manufactured by Perceptive Instruments, Comet Assay IV Now Offered Through Instem for Assessing DNA Damage with Live Video-based System
Detection of cytogenetic and DNA damage in peripheral erythrocytes of goldfish (Carassius auratus) exposed to a glyphosate formulation using the micronucleus test and the comet assay.