Putative association between a new polymorphism in exon 3 (Arg109Cys) of the pancreatic colipase
gene and type 2 diabetes mellitus in two independent Caucasian study populations.
Secretion of pancreatic lipase and colipase
from rat pancreas.
2) However, contemporary lipase assays now contain colipase
and bile salts that increase the analytical sensitivity of lipase assays.
All four experimental methods (experimental reagents from Sentinel) contained colipase, but differed from one another in stabilizing proteins and in the size of substrate micelles: two methods contained protein (0.
Our data show that, for the lipase assays that use 1,2-o-dilauryl-rac-glycero-3-glutaric acid- (6'-methylresorufin) ester as substrate, the frequency of noncommutability may be affected by some characteristics of the analytical method, such as size of substrate micelles and the presence of stabilizing proteins different from colipase.
Serum lipase activity increased in all our patients without a parallel increase in serum amylase titers; the combination of accurate clinical, biochemical, and instrumental study of these patients allowed us to exclude the possibility of a normoamylasemic pancreatic injury, and the presence of appropriate concentrations of bile salts and colipase
in the reagent favors a full and specific action of lipase assay, at the same time inhibiting serum esterase (8).
In the small intestine lipolysis is completed by pancreatic lipase acting with colipase
as cofactor and absorption of lipolytic products starts.