colcemid


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Colcemid

 [kol´sĕ-mid]
trademark for a preparation of demecolcine, a cytotoxic alkaloid used in chromosome analysis.

colcemid

a compound related in structure and function to colchicine.
References in periodicals archive ?
Then, metaphases are harvested by adding colcemid (10 mg/l) for 120 min, followed by hypotonic KCl (0.
2] por 48 horas; 90 minutos antes de la cosecha, se anadio Colcemid 0,2 ug/mL.
2 ml of colcemid was added to the culture, two hours prior to harvest i.
Harvest: Initially 70 [micro]L of 10 [micro]g/ml vial of colcemid was added to the sample and incubated in 37[degrees]C for 20 min.
12], with the incubation of cultures during 72 hours and the application of Colcemid stock solution 25 mcg/ml (0.
1 mg/mL colcemid (Sigma, Santa Clara, USA) for 4 h at 37C, then harvested, centrifuged and resuspended in 0.
Lymphocytes were cultured in the dark for 48 h and metaphases were blocked during the last 2 h with colcemid (Biological Industries, Beit Haemek, Israel) at a final concentration of 0.
En estudios citogeneticos se comparo el efecto de la colchicina, el colcemid y la vinblastina, cada uno a cinco concentraciones diferentes (0.
BM samples were cultured for 24-48 hours in RPMI-1640 medium (supplemented with %10 fetal calf serum and antibiotics) without mitogen stimulation and with 10[micro]g/ml colcemid concentration.
The culture was synchronized by the addition of Colcemid at 72 hrs.
High-resolution cultures were initiated in RPMI media with GlutaMAX (Gibco) and treated with 5-fluoro-2'-deoxyuridine 48 hours after culture initiation and ethidium bromide and colcemid at the time of harvest.