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coding sequence |
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The complete coding sequence of AHFV-JE7 was determined by amplification and sequencing of overlapping PCR products by using the long PCR product sequencing strategy (6) (primers and detailed protocol available on request). The team also studied the importance of tryptophan residues to p19's silencing suppression effects by introducing mutations into the p19 coding sequence of the CIRV genome to change one or both of the tryptophan residues. Identification of the complete coding sequence and genomic organization of the Treacher Collins syndrome gene. |
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