suis in 38 patients; all isolates were serotype 2 (PCR and coagglutination
results were all concordant).
Use of polyvalent coagglutination
reagents for serotyping of Streptococcus suis.
The final serotype of all strains was confirmed by coagglutination
tests that used rabbit antiserum (Statens Serum Institute, Copenhagen, Denmark).
The isolate was assigned to serotype 2 by the coagglutination
test (7) and shown by Western blot to produce suilysin, extracellular protein factor and muramidase-released protein, which are virulence markers often associated with highly virulent strains of Eurasian, but not North American, origin (1,8).
CAMP Test, Serotyping, Antimicrobial Susceptibility, and Pulsed-Field Gel Electrophoresis Identification of the isolates was confirmed by CAMP testing, and serotype determination was achieved by using a coagglutination
typing kit (Essum, Bacterium AB, Umea, Sweden).
suis serotyping, where it was identified by the coagglutination
test as serotype 14 (5).
Serotyping and subtyping of Neisseria meningitidis isolates by coagglutination
, dot blotting and ELISA.
Isolates were serotyped by the Quellung reaction (Alaska, northern Canada, Greenland, Norway, and northern Sweden), counter-immunoelectrophoresis (Finland), or coagglutination
Serotyping by coagglutination
tests showed that 19 of the 20 isolates belonged to serotype 2, while the remaining 1 (MNCM07) was serotype 14.
suis antisera specific against individual serotypes (provided by the Statens Serum Institut, Copenhagen, Denmark) and confirmed by the coagglutination
test, as previously reported (12).