The clonogenic assay
has been described in detail (Hajji et al.
The presence of CSCs is usually inferred on the basis of one of the following characteristics: (a) flow cytometry results indicating the presence of "specific" cell surface markers; (b) clonogenic assay
findings such as mammosphere formation; and (c) ability of cells to give rise to metastases in xenotransplantation models (6).
Cytotoxic effect of alloy specimens was evaluated by performing Cell viability assay and Clonogenic assay
Growth inhibition, in combination with radiotherapy, was assessed using a clonogenic assay
Using advanced research tools that combine reporter mice, clonogenic assays
, organotypic cultures, high-throughput RNAi screen and genome-wide epigenetic and transcriptomic profiling, we will dissect the principles that underlie the self-renewal and lineage differentiation of TEC progenitors in vivo.
Br2DR cells showed a slight increased sensitivity to IR and MMC based on clonogenic assays
in contrast to the exquisite radiosensitivity and chemosensitivity of cells derived from patients that have defects in DNA damage sensing and repair proteins.
were utilized to assess response.
These additions to Sigma-Aldrich's growing portfolio of research tools for regenerative medicine are expected to consist of serum-free media for bone marrow and umbilical cord stem cells, mesenchymal stem cells and neural stem cells; cytokine cocktails for stem cell expansion and differentiation; novel formulations for cyropreservation of stem cells; and specialized products to conduct clonogenic assays
of the various stem cells (http://www.
Significant radiation enhancement was also observed in clonogenic assays
under HO at clinically relevant doses, raising the potential for synergistic and dose-sparing radiation therapy.
Discordance in the literature that investigates the chemopreventive efficacy of anethole and its contradicting estrogenic activity Derivatives of anethole have demonstrated anethole might act as a growth promoting or growth inhibitory compound, we were interested in the effect To determine whether trans-anethole has any impact on the cell survival, we conducted clonogenic assays
using MCF-7 and MDA-MB-231 in which cell were treated with increasing (1 x [10.
In vitro assessment of Ara-C efficacy has previously involved measurement of cell death, assessed by colony forming units-blast clonogenic assays
The CD34-selected cord blood clonogenic assays
with recombinant BMP-2 is depicted in Figure 3.