cloning vector


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Related to cloning vector: expression vector

clon·ing vec·tor

an autonomously replicating plasmid or phage with regions that are not essential for its propagation in bacteria and into which foreign DNA can be inserted; this foreign DNA is replicated and propagated as if it were a normal component of the vector.
A recombinant DNA molecule containing sequences from 2 or more sources—e.g., plasmids, bacteriophage, cosmids—into which foreign DNA—40+ kb—may be inserted for cloning; a DNA sequence capable of self-replication in a suitable host cell, which has site(s) for inserting DNA fragments by recombinant DNA techniques, and genetic markers that allow selection for the vector in a host cell; CVs usually have a selective marker—e.g., for antibiotic resistance—and a site for cleavage by a restriction endonuclease

clon·ing vec·tor

(klōn'ing vek'tŏr)
An autonomously replicating plasmid or phage with regions that are not essential for its propagation in bacteria and into which foreign DNA can be inserted; this foreign DNA is replicated and propagated as if it were a normal component of the vector.

cloning vector

see VECTOR.

vector

1. a carrier, especially the animal (usually an arthropod) which transfers an infective agent from one host to another, e.g the tsetse fly, which carries trypanosomes from animals to humans, dogs, bats and other animals that transmit the rabies virus. In molecular biology, a DNA molecule which serves to transfer DNA into a host cell.
2. a quantity possessing magnitude, direction and sense (positivity or negativity).

biological vector
an arthropod vector in whose body the infecting organism develops or multiplies before becoming infective to the recipient individual.
cloning vector
a DNA molecule used to transfer an inserted DNA segment into a host cell. Includes other viruses, phages and bacterial plasmids. Called also cloning vehicle.
mechanical vector
an arthropod vector that transmits the infective organisms from one host to another but is not essential to the life cycle of the parasite.
shuttle v's
vectors which contain both prokaryotic and eukaryotic replication signals, thus allowing replication of the vector in both kinds of cells.
targeting vector
a vector carrying a DNA sequence that is able to take part in a specified chromosomal crossover in the host.
References in periodicals archive ?
The purified PCR products were ligated into pMD-18T cloning vector (Takara Biotechnology, Dalian, China), transformed and sub-cultured.
The molecular mechanism for blue/white screening is based on genetic engineering of the lac operon in the Escherichia coli laboratory strain serving as a host cell, combined with a subunit complementation achieved with the cloning vector.
The claims are directed to a modified glyphosate resistance gene, cloning vectors containing the gene, various plant cells containing the gene, and a process for inserting the gene into cells.
The amplified DNA was then prepared for insertion into cloning vectors by restriction with BarnHI and NdeI.
Scientists have been attempting to construct a variety of food-grade cloning vectors for use in lactic acid bacteria and to improve the bacteriophage resistance of dairy starter cultures using genetic engineering techniques.
Quality-controlled, consistent frozen cell stocks, cloning vectors and standardized media for algal model organisms Chlamydomonas reinhardtii and Synechococcus elongatus
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
The goals of research at the Cooperative Research Center for Food Industry Innovation (CRC, University of New South Wales, Biotechnology Department, Sydney NSW 2052, Australia) are to construct a variety of food-grade cloning vectors for use in LAB and to improve the bacteriophage resistance of dairy starter cultures using genetic techniques.
Invitrogen offers numerous TOPO Cloning vectors for a variety of applications including vectors for expression in bacterial, yeast, insect and mammalian cells.
Davis created some of the earliest cloning vectors, DNA molecules that carry foreign DNA into a host cell where it can be replicated.