cloning vector


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Related to cloning vector: expression vector

clon·ing vec·tor

an autonomously replicating plasmid or phage with regions that are not essential for its propagation in bacteria and into which foreign DNA can be inserted; this foreign DNA is replicated and propagated as if it were a normal component of the vector.
A recombinant DNA molecule containing sequences from 2 or more sources—e.g., plasmids, bacteriophage, cosmids—into which foreign DNA—40+ kb—may be inserted for cloning; a DNA sequence capable of self-replication in a suitable host cell, which has site(s) for inserting DNA fragments by recombinant DNA techniques, and genetic markers that allow selection for the vector in a host cell; CVs usually have a selective marker—e.g., for antibiotic resistance—and a site for cleavage by a restriction endonuclease

clon·ing vec·tor

(klōn'ing vek'tŏr)
An autonomously replicating plasmid or phage with regions that are not essential for its propagation in bacteria and into which foreign DNA can be inserted; this foreign DNA is replicated and propagated as if it were a normal component of the vector.

cloning vector

see VECTOR.

vector

1. a carrier, especially the animal (usually an arthropod) which transfers an infective agent from one host to another, e.g the tsetse fly, which carries trypanosomes from animals to humans, dogs, bats and other animals that transmit the rabies virus. In molecular biology, a DNA molecule which serves to transfer DNA into a host cell.
2. a quantity possessing magnitude, direction and sense (positivity or negativity).

biological vector
an arthropod vector in whose body the infecting organism develops or multiplies before becoming infective to the recipient individual.
cloning vector
a DNA molecule used to transfer an inserted DNA segment into a host cell. Includes other viruses, phages and bacterial plasmids. Called also cloning vehicle.
mechanical vector
an arthropod vector that transmits the infective organisms from one host to another but is not essential to the life cycle of the parasite.
shuttle v's
vectors which contain both prokaryotic and eukaryotic replication signals, thus allowing replication of the vector in both kinds of cells.
targeting vector
a vector carrying a DNA sequence that is able to take part in a specified chromosomal crossover in the host.
References in periodicals archive ?
Firstly, this plasmid was digested with two restriction enzymes including NdeI and BamHI-HF (BioLabs (UK)), for separation of insulin-like growth factor type 1 gene from pUC18 cloning vector.
In order to confirm that cellulase gene was successfully inserted in the pTZ57R/T cloning vector, colony PCR was performed.
The only difference between the Set 1 and 2 sense plants was the additional fragment of the cloning vector in the construct of the Set 1 plants.
The amplified fragments of exochitinase A gene were ligated in pTZ57R/T cloning vector and were used for transformation of E.
After size-fractioning of products in agarose gels, each product was purified (Wizard[R] SV gel and PCR clean-up system --Promega) and cloned into pGEM-T Easy Vector Kit (Promega), which has a plasmid of 3015 bp as cloning vector and a cleavage site for each of the restriction enzymes used in the analysis (in the linker), including two EcoRI sites flanking the PCR product cloning site.
The purified PCR product was ligated with the pDrive cloning vector and then transformed into the QIAGEN EZ competent cell.
Kazuki Saito, Chiba University, Chiba, Japan) was transformed with pSCS10 and the cloning vector pET-28a served as a negative control.
Then, the DNA fragments were ligated into the cloning vector pUC118 which had been previously digested by BamHI and dephosphorylated with calf alkaline phosphatase (TaKaRa, Dalian).
The 16S rRNA gene was amplified by 27 forward and 1492 reverse primers and cloned using TA cloning vector.
Methods: LC and Hc c gene segments were amplified from Clostridium tetani genomic DNA by PCR, cloned into pET28b(+) cloning vector and transformed in Escherichia coli (E.
Patent and Trademark Office for a patent application entitled Stem-Loop Cloning Vector And Method.