clon·ing (kl  n ng)n. The transplantation of a nucleus from a somatic cell into an ovum, which then develops into an embryo. |
cloning
[klō′ning]
a procedure for producing multiple copies of genetically identical organisms or cells or of individual genes. Organisms may be cloned by transplanting blastocysts from one embryo into an empty zona pellucida, or nuclei from the cells of one individual into enucleated oocytes. Cells may be cloned by growing them in culture under conditions that promote cell reproduction. Genes may be cloned by isolating them from the genome of one organism and incorporating them into the genome of an asexually reproducing organism, such as a bacterium or a yeast.
cloning
see recombinant DNA technology.
directional cloning
the insertion of a segment of foreign DNA which has a defined polarity, e.g. different restriction enzyme sites at each end, into a plasmid vector.
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