Chitinoclastic bacteria comprised a very small traction of bacteria present in the lesions, suggesting that their role in epizootic shell disease may be limited.
We focused, however, on chitinoclastic bacteria able to use crude chitin.
During this long incubation period, many gliding and swarming bacteria would cover the agar, sometimes completely obscuring halos formed around colonies of chitinoclastic bacteria.
We also analyzed by RFLP 16S rRNA genes from the isolated chitinoclastic bacteria as well as bacteria isolated from hemolymph.
For 16S rDNA sequence analysis, we selected to sequence portions of the 16S rRNA genes from all bacteria isolated from the lobsters ELIS6 and ELIS10 as representatives, including both "common" and "rare" isolates 16S rRNA genes from all chitinoclastic isolates and most bacteria isolated from hemolymph were also sequenced.
Chitinoclastic bacteria forming yellow colonies belong to one of the three closely related species, which we propose to assign tentatively to "the lobster Flavobacteriaceae species complex.