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Pertaining to chaotropism.
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Given our observed inability to greatly alter soy flour adhesion using chaotropic agents, salts, surfactants, or co-solvents (Frihart and Lorenz 2013), which is in contrast to the literature observations with soy protein isolate (Sun 2005b), we concluded that the carbohydrates were suppressing protein alteration for improved adhesion.
Lysis is currently accomplished through application of pressure, heat, chemicals (1) (including chaotropic salts to be used later in purification), sonication, (2) and/or mechanical lysis (3) (for instance using zirconium (3) or silica (2) or ceramic (4-5) beads).
Known inactivation methods using surfactants or chaotropic salts denature both chains of the hololectin.
Many of the established RNA isolation protocols are based on highly toxic chaotropic agents such as phenol, phenol/chloroform or guanidine thiocyanate which quickly denature the endogenous ribonucleases in tissues [8].
Disruption of most cells is done by chaotropic salts, detergents or alkaline denaturation, and the resulting lysate is usually cleared by centrifugation, filtration or magnetic clearing.
tularensis isolates were recultured, then a loopful of bacteria was suspended in phosphate-buffered saline and heat-killed, and a chaotropic salt method was applied (6).
1]-dissociation during treatment with chaotropic salts in contrast to the former where it dissociates easily and is a sensitive target of cold stress [86].
According to Hofmeister series, ions are either kosmotropic (they tend to destabilize the hydrogen bonds between water molecules or between water and polymeric chains leading to a collapsing of the polymeric network) or chaotropic (they tend to stabilize the hydrogen bonds in hydrophilic polymers leading to polymeric matrix swelling) (Bagger et al.
Firstly, a chaotropic agent like guanidium chloride was added to unfold the proteins.
The BOOM technology combines the lysing and nuclease-inactivating qualities of chaotropic salt buffer with the nucleic acid binding quality of silica," says Frost & Sullivan senior research analyst Sachin Thukral.
Separations in multiple-channel microchips, temperature effects in liquid chromatography, the use of polysaccharide phases in the separation of enantiomers, and chaotropic effects in RP-HPLC are some of the essay topics.