Given our observed inability to greatly alter soy flour adhesion using chaotropic
agents, salts, surfactants, or co-solvents (Frihart and Lorenz 2013), which is in contrast to the literature observations with soy protein isolate (Sun 2005b), we concluded that the carbohydrates were suppressing protein alteration for improved adhesion.
Lysis is currently accomplished through application of pressure, heat, chemicals (1) (including chaotropic
salts to be used later in purification), sonication, (2) and/or mechanical lysis (3) (for instance using zirconium (3) or silica (2) or ceramic (4-5) beads).
Known inactivation methods using surfactants or chaotropic
salts denature both chains of the hololectin.
Many of the established RNA isolation protocols are based on highly toxic chaotropic
agents such as phenol, phenol/chloroform or guanidine thiocyanate which quickly denature the endogenous ribonucleases in tissues .
Total RNA and DNA were bound to a fresh volume of beads under chaotropic
conditions in the deep-well plate.
Disruption of most cells is done by chaotropic
salts, detergents or alkaline denaturation, and the resulting lysate is usually cleared by centrifugation, filtration or magnetic clearing.
tularensis isolates were recultured, then a loopful of bacteria was suspended in phosphate-buffered saline and heat-killed, and a chaotropic
salt method was applied (6).
1]-dissociation during treatment with chaotropic
salts in contrast to the former where it dissociates easily and is a sensitive target of cold stress .
According to Hofmeister series, ions are either kosmotropic (they tend to destabilize the hydrogen bonds between water molecules or between water and polymeric chains leading to a collapsing of the polymeric network) or chaotropic
(they tend to stabilize the hydrogen bonds in hydrophilic polymers leading to polymeric matrix swelling) (Bagger et al.
Firstly, a chaotropic
agent like guanidium chloride was added to unfold the proteins.
The BOOM technology combines the lysing and nuclease-inactivating qualities of chaotropic
salt buffer with the nucleic acid binding quality of silica," says Frost & Sullivan senior research analyst Sachin Thukral.
Separations in multiple-channel microchips, temperature effects in liquid chromatography, the use of polysaccharide phases in the separation of enantiomers, and chaotropic
effects in RP-HPLC are some of the essay topics.