It catalyzes the hydrolysis of arly and alkyl glycosides as well as of cellobiose
to glucose which is inhibitory to the endo and exo cellulases during cellulose hydrolysis .
Saratale and Oh (2011) reported that the decrease in cellulolytic enzyme production at the stationary phase was caused by metabolite repression by molecules released after the hydrolysis such as glucose or cellobiose
Altogether 12 saccharides and sugar alcohols (glucose, galactose, fructose, arabinose, xylose, ribose, fucose, cellobiose
, glucitol, mannitol, arabitol, and myoinositol) were identified using capillary electrophoresis.
It was incapable of growing in the absence of NaCl and did not produce acid from D-glucose, Dmannose, D-fructose, maltose, D-mannitol, rhamnose, sucrose, D-trehalose, D-galactose, cellobiose
, Draffinose, xylose, lactose, ribose, arabinose, D-melezitose, D-salicin and aesculin.
Sugars C3 A2 A3 A4 A5 Esculine Hdrolysis -ve +ve +ve +ve +ve Arabinose -ve +ve +ve -ve +ve Xylose -ve -ve -ve -ve -ve Adonitol -ve -ve -ve -ve -ve Rhomnose -ve -ve -ve +ve -ve Cellobiose
-ve -ve +ve +ve +ve Melibinose -ve +ve -ve +ve -ve Raffinose -ve +ve +ve +ve -ve Trehalose -ve +ve +ve +ve +ve Glucose -ve +ve +ve +ve +ve Lactose -ve +ve -ve +ve -ve Sacarose -ve +ve +ve +ve +ve
Carbohydrate DM15 * 1 Xylose + 2 Trehalose + 3 Mellibiose ++ 4 Galactose ++ 5 Mannitol + 6 Sorbitol - 7 Raffinose ++ 8 Cellobiose
+++ 9 Arabinose - 10 Fructose +++ 11 Maltose +++ 12 Lactose +++ 13 Rhamnose - 14 Glucose +++ 15 Sucrose ++ 16 Glycerol - * (+++): strongly positive, (++): fairly positive, (+): weakly positive, (-): negative Table 3: Antibiotic sensitivity of the isolate DM15.
The key is the strain's ability to ferment cellobiose
and galactose simultaneously, which makes the process much more efficient," he said.
In comparison with other commercially available cellulases, Accellerase 1500 contains higher levels of beta-glucosidase activity, which ensures almost a complete conversion of cellobiose
to glucose and faster ethanol fermentation.
Classical mutations of the cellulase-producing fungus Trichoderma reesei, which was discovered during World War II, improved the enzymes by, for example, enhancing beta-glucosidase activity for converting cellobiose
, a powerful inhibitor, into glucose.
Determination of inhibition in the enzimatic hydrolysis of cellobiose
using hybrid neural modeling.
2] with 1% NaCI 75 9 Motility 99 98 Acid from: sucrose 100 0 d-mannitol 99 99 cellobiose
8 0 salicin 1 0 Group 5 Test V damsela Nutrient broth (1) No NaCI -- 1 % NaCI + V[P.
These carbohydrates include glucose, mannose, galactose, xylose, arabinose, fructose, sorbose, lactose, sucrose, cellobiose
, cellotriose, and raffinose.