Capillary isoelectric focusing and high-performance cation-exchange chromatography
compared for qualitative and quantitative analysis of hemoglobin variants.
The pellets were reconstituted in urea, and samples were subjected to trypsin digestion and strong cation-exchange chromatography
Consequently, changes or mutations in the Hb molecule that result in a charge difference from the wild-type Hb species, may or may not alter the normal elution time of those Hb species when using cation-exchange chromatography
Step 4: Cation-exchange chromatography
was carried Out on a column of SP Sepharose High Performance (Pharmacia; 1 cm X 6 cm) at room temperature.
The primer-extension products were dried under reduced pressure and then desalted by use of sequential POROS and cation-exchange chromatography
2] fraction of a heterozygous HbS carrier 3 times by cation-exchange chromatography
, and MALDI-TOF analysis revealed the peak at 15 838 Da.
The first group of methods, which includes cation-exchange chromatography
and electrophoresis, separates glycated from nonglycated hemoglobin components based on differences in their charges.
The mobile phases for cation-exchange chromatography
were a solution of 20 mmol/L pyridine in water, pH 2.
Gas chromatographic-mass spectrometric determination of urinary oxoacids using 0-(2,3,4,5,6-pentafluorobenzyl) oxime-trimethylsilyl ester derivatization and cation-exchange chromatography
Boronate affinity or affinity-binding chromatography, cation-exchange chromatography
, and immunoassays form the most common means for measuring gHb.
0], both purified by cation-exchange chromatography
and affinity chromatography (9).
One comparison (5) of CIEF with cation-exchange chromatography
for the qualitative and quantitative analysis of Hb variants found that quantitative data between the two methods were highly correlated and that CIEF gave slightly better resolution of the unusual variants Hb C Harlem and Hb D Punjab.