CASP7

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CASP7

A gene on chromosome 10q25 that encodes a protein belonging to the cysteine-aspartic acid protease (caspase) family which, once activated by proteolytic processing, plays a central role in the execution phase of apoptosis. Effector (also called executioner) caspases CASP3, -6 and -7 are responsible for cleaving downstream substrates—CASP7 cleaves and activates sterol regulatory element binding proteins (SREBPs). It is highly expressed in the lung, skeletal muscle, liver, kidney, spleen and heart.
References in periodicals archive ?
Caspase-7 deficiency protects from endotoxin-induced lymphocyte apoptosis and improves survival.
The protective effects shown by caspase inhibitors are further supported by experiments using selective caspase-7 deficient mice that were found to be resistant to lipopolysaccharide-induced lymphocyte apoptosis as well as lipopolysaccharide-induced lethality, independent of the excessive production of serum cytokines (44).
Both caspase-3 and caspase-7 cleavage PARP resulting in the formation of the 85 kDa fragment.
Caspase-3, caspase-7 and caspase-8 were expressed in Escherichia coli and isolated in the full active form as previously described (Stennicke and Salvesen, 1999).
Murray (Polygonaceae)) blocked the activity of the initiator caspase-8 (Kruidering and Evan, 2000) and the effector caspases caspase-3 (Porter and Janicke, 1999), and caspase-7 (Korfali et al.
We found that aqueous extracts of Lianqiao (Fructus Forsythiae) and Shouwuteng (Caulis Polygoni multiflori) blocked the activity of the initiator caspase-8 as well as the effector caspase-3 and caspase-7 in a dose-dependent manner with an I[C.
Proapoptotic stimuli first lead to the activation of so-called initiator caspases such as caspase-8, which in turn cleave inactive proenzyme forms of so-called effector caspases such as caspase-3 and caspase-7.
As a consequence, cleaved caspase-9 and cleaved caspase-7 levels were significantly downregulated, indicating negative regulation of apoptosis by COC extract.
Immunoblot analyses of caspase-9, caspase-8, caspase-7, caspase6, caspase-3, PARP and MAPKs and isolation of mitochondria and release of cytochrome c from mitochondria were performed as described previously (Torres et al.
Antibodies against [alpha]-tubulin (Oncogene Science), phospho-Cdkl (BioSource International), phospho-Chk2 (R&D Systems), caspase-7 and cytochrome c (BD Pharmingen), phospho-ATM (Cell Signaling Technology), proliferating-cell nuclear antigen (PCNA) (Transduction Laboratories) were purchased as indicated, all other antibodies were from Santa Cruz Biotechnology (Santa Cruz CA).
The enzymatic activity of caspase-7 in vehicle or taiwanin A-treated MCF-7 cells was also measured.
B) Caspase-7 activity in the whole cell lysates prepared from MCF-7 cells with or without taiwanin A treatment was determined.
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