CASP7

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CASP7

A gene on chromosome 10q25 that encodes a protein belonging to the cysteine-aspartic acid protease (caspase) family which, once activated by proteolytic processing, plays a central role in the execution phase of apoptosis. Effector (also called executioner) caspases CASP3, -6 and -7 are responsible for cleaving downstream substrates—CASP7 cleaves and activates sterol regulatory element binding proteins (SREBPs). It is highly expressed in the lung, skeletal muscle, liver, kidney, spleen and heart.
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Assessment of apoptosis by immunohistochemistry to active caspase-3, active caspase-7, or cleaved PARP in monolayer cells and spheroid and subcutaneous xenografts of human carcinoma.
Different sub-cellular distribution of Caspase-3 and Caspase-7 following Fas-induced apoptosis in mouse liver.
VSMCs, indicating that there is no residual caspase-7 activity in caspase-3 deficient BMDM and VSMCs.
VSMCs based on the absence of typical apoptotic morphological changes and lack of cleavage of caspase-3 and caspase-7 (data not shown).
Executioner caspase-3 and caspase-7 are functionally distinct proteases.
The protective effects shown by caspase inhibitors are further supported by experiments using selective caspase-7 deficient mice that were found to be resistant to lipopolysaccharide-induced lymphocyte apoptosis as well as lipopolysaccharide-induced lethality, independent of the excessive production of serum cytokines (44).
Caspase-7 deficiency protects from endotoxin-induced lymphocyte apoptosis and improves survival.
As a consequence, cleaved caspase-9 and cleaved caspase-7 levels were significantly downregulated, indicating negative regulation of apoptosis by COC extract.
Both caspase-3 and caspase-7 cleavage PARP resulting in the formation of the 85 kDa fragment.
Immunoblot analyses of caspase-9, caspase-8, caspase-7, caspase6, caspase-3, PARP and MAPKs and isolation of mitochondria and release of cytochrome c from mitochondria were performed as described previously (Torres et al.
Antibodies against [alpha]-tubulin (Oncogene Science), phospho-Cdkl (BioSource International), phospho-Chk2 (R&D Systems), caspase-7 and cytochrome c (BD Pharmingen), phospho-ATM (Cell Signaling Technology), proliferating-cell nuclear antigen (PCNA) (Transduction Laboratories) were purchased as indicated, all other antibodies were from Santa Cruz Biotechnology (Santa Cruz CA).
We found that aqueous extracts of Lianqiao (Fructus Forsythiae) and Shouwuteng (Caulis Polygoni multiflori) blocked the activity of the initiator caspase-8 as well as the effector caspase-3 and caspase-7 in a dose-dependent manner with an I[C.
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