A calibration curve
was prepared for developing and validating the analytical method.
These samplings present high cost and low efficiency, since the amplitude of SSC is not satisfactory to construct a calibration curve
, requiring extrapolation to samples with higher turbidity, increasing the chances of error.
The limit of detection (LOD) and limit of quantitation (LOQ) for the 20 elements in maize flour were determined for each calibration curve
by using the following equations: LOD = 3*SD/S, LOQ = 10*SD/S, where, SD is the standard deviation of a response, and S is the slope of the calibration curve
(Shrivastava and Gupta, 2011).
Amount of lactose in camel milk solutions were determined using the standard calibration curve
made for lactose 
Analysis of G, A, C and T from acid hydrolyzed DNA using linear calibration curve
with spiked samples
The calibration curve
was measured in an ex-situ experiment following the same experimental procedures explained in previous work .
Preparation of calibration curves
is often more an exercise in curve preparation than a useful evaluation of patient sample accuracy and imprecision, which is best evaluated by QC samples prepared identically to patient samples.
data, LOD, LOQ, and recoveryvalues are listed in Table 4.
TotalCal allows for four NIST (National Institute of Standards and Technology) calibration films to be stored and a calibration curve
is generated across the system's entire range.
In order to minimize the large offset and gain errors that remain after the application of the same Steinhart-Hart equation for all the sensors from the same batch, the calibration curve
for each thermistor of the thermistor group was corrected by using additional data obtained after measuring the resistance of each thermistor of the group at the two points (adjacent to the limits of the temperature range).
A standard calibration curve
was generated by spiking different aliquots of the working stock solution into blank human urine specimen; yielding a nine-point calibration curve
Figure 3 shows the calibration curve
for NYS I solutions in [[lambda].