Our case showed positive staining of tumor cells, a diffuse staining with actin, desmin and caldesmon
and focal with HMB-45 and melan-A.
Immunohistochemically, the tumor cells were positive for vimentin, sex determining region of Y chromosome-related high-mobility group box gene-9 (SOX-9), S-100, P16, murine double minute 2 (MDM2), and cyclin-dependent kinase 4 (CDK4) and negative for pan-cytokeratin (PCK), epithelial membrane antigen (EMA), smooth muscle actin (SMA), desmin, myogenin, cluster of differentiation 10 (CD10), CD31, CD34, caldesmon
, CD99, beta-catenin, signal transducer, and activator of transcription 6 (STAT-6).
(CaD)-like protein was purified from Physarum (Table 7).
Differentiated or contractile VSMCs express unique markers, such as smooth muscle myosin heavy chain (SM-MHC), smooth muscle [alpha]-actin, transgelin, high molecular weight caldesmon
) and calponin, all of which are crucial for vasoregulation.
In the present case, immunohistochemical staining showed that actin (+), vimentin(+) and caldesmon
(+) were found to be positive.
Histological examination of a renal leiomyoma reveals fusiform to spindle shaped cells with positive immunohistochemical staining for smooth muscle markers (desmin, caldesmon
, and SMA) and negative staining for HMB-45 and cathepsin K.
Phosphorylation of smooth muscle caldesmon
by mitogen-activated protein (MAP) kinase and expression of MAP kinase in differentiated smooth muscle cells.
Antibody Manufacturer Type Clone Isotype Code CK7 Dako MMAH OV-TL IgG1, M7018 12/30 kappa Desmin Dako MMAH D33 IgG1, M0760 kappa EMA Dako MMAH E29 IgG2a, M0613 kappa Melan-A Dako MMAH A103 IgG1, M7196 kappa S100 Dako Polyclonal - - Z0311 rabbit anti-S100 HMB-45 Dako MMAH HMB-45 - IR05 SMA Dako MMAH 1A4 IgG2a, M0851 kappa Caldesmon
Dako MMAH h-CD IgG1, M3557 kappa CD34 Dako MMAH QBEnd IgG1, M7165 10 kappa CD31 Dako MMAH JC70A IgG1, M0823 kappa Factor Dako Polyclonal - - IR527 VIII rabbit anti-human CK20 Dako MMAH Ks20.
Immunohistochemical studies demonstrated the expression of vimentin, smooth muscle actin (SMA), actin (HHF35), and caldesmon
The mesenchymal component was positive for vimentin and CD 10, focally positive for CD68 and factor XIII, and negative for cytokeratin, S-100, caldesmon
, and smooth-muscle actin (figure 3, B).
Purification and characterization of erythrocyte caldesmon