calcein


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calcein

A chelating agent which fluoresces brightly in the presence of bound Ca2+. The acetomethoxy derivative of calcein is used as a temporary label to test cell viability: once the calcein acetomethoxy complex enters healthy cells, intracellular esterases remove the acetomethoxy group, which fluoresces bright green—apparently in protest—something that dead cells, which lack esterases, cannot do.
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Finally, 8 [micro]g/ml Calcein AM (BD Biosciences) was added to stain microvessels.
2003) analyzed the effect of strontium and calcein on the growth pattern in Ruditapes philippinarum (Adams & Reeve, 1850), and reported that strontium is better for estimating age than calcein if the organism has a long life span.
We obtained fluorescein isothiocyanate (FITC)-labeled annexin V (annexin V-FITC) from Pharmingen (San Diego, CA, USA) and 5-(-6)-carboxyfluorescein diacetate (CFDA), calcein red-orange, anti-CD13-PE, anti-CD13-perCP-Cy5.
The devices were observed under a fluorescence microscope to determine the number of viable GFP-labeled cells that were positive for the Calcein dye (blue) and negative for the ethidium homodimer-1 (red).
4), and viability was assessed by the addition of 25 [micro]M calcein AM.
Effects of individual inhibitors and protease cocktails are compared 1[micro]M of Calcein AM and 2[micro]M of Ethidium Bromide are the optimal concentrations for the live dead viability assay.
When the dendrimers interacted with small unilamellar vesicles (SUV) loaded with the self-quenching fluorophore calcein they did not induce release of calcein neither with neutral SUV (made of egg phosphatidylcholine) nor negatively charged SUV (with 25% egg phosphatidylglycerol).
6] M) calcein solutions with varying Ni(II) concentrations (0-[10.
2]DCF, CMF and calcein were acquired in the FL1 channel, equipped with a 530/30 nm band pass filter, PI in the FL2 channel having a 585/42 nm band pass filter and NAO in the FL3 channel having a 682/33 nm band pass filter.
Calcein has been shown to be a reliable marker in bivalve shells and to cause low mortality (Day et al.
Chondrocytes were seeded and their viability was tested by fluorescence with calcein green.