Predicted amino acid sequence of recombinant ISG15 cDNA
When we analyzed the products of second-strand cDNA
synthesis, the majority of the nucleic acids in the samples were single-stranded nucleic acids (Fig.
Synthesis of second-strand cDNA
and amplification of genespecific cDNA
(Figure 1B) were performed by adding 2 [micro]L of the RT-cDNA
, 1 [micro]L forward primer, 1 [micro]L reverse primer (50 pmoles each), and 12.
Four hundred and forty-nine clones were dot-blotted on 18 x 12 cm nylon membrane using manual microarrayer, and probed with reverse transcribed DIG-labeled cDNA
Isolation of full-length putative rat lhysophospholipase cDNA
using improved methods for mRNA isolation and cDNA
Each of the pPICZ[alpha]A-modified huIFN[alpha]2 plasmids (FS, WS and SW) was cut according to the manufacturer's instructions using SacI, which does not cut within the modified IFN[alpha]2 cDNA
The supernatant was transferred to a fresh tube, the cDNA
reamplified using the same primers and similar PCR conditions except radio-labeled dATP was not used and the dNTP concentrations were increased to 250 [micro]M.
The plasmid DNA of clones ShIL1-21 and ShIL1-28 were digested with Eco RV and Not I (Promega), ran in a 1% agarose gel and the cDNA
inserts were eluted using Spin-X columns.
The team's original cDNA
libraries came from a single switchgrass variety.
Access to Kazusa's collection of cDNA
is essential to creating focused maps of important human diseases -- ultimately leading to the faster development of safer, more targeted medicines.
Using suppression PCR cDNA
subtractive hybridization between regenerating larvae and uncut controls, we identified 9 cDNA
fragments representing early regeneration-specific gene expression.
Susan Russell of the CDNA
said: "No nurse should have to transport any form of clinical waste - it is a health risk to their families.