The 3 segmented RNAs of Bunyamwera virus
were used as outgroups to root the trees.
harbored a previously unidentified member of the Bunyamwera virus
The largest genus in the family Bunyaviridae, the genus Orthobunyavirus was originally named Bunyavirus, for the type species Bunyamwera virus
, first isolated in 1943 from the eponymous town in western Uganda.
Ngari virus is a Bunyamwera virus
reassortant that can be associated with large outbreaks of hemorrhagic fever in Africa.
Sequence analysis showed that NRIV is a reassortant between Bunyamwera virus
(BUNV) and Batai virus (BATV), both from the genus Orthobunyavirus.
Serum specimens were tested for immunoglobulin M (IgM) and IgG antibodies specific for DENV, West Nile virus (WNV), Wesselsbron virus, Rift Valley fever virus, Bunyamwera virus
, and CHIKV by IgM-antibody capture (MAC-ELISA) and IgG sandwich ELISA, respectively (11).
The first sample (November 3, 2004) contained no antibodies (OD ratio <2) to dengue viruses, West Nile virus, Wesselsbron virus, Rift Valley fever virus, Bunyamwera virus
, or Chikungunya virus (CHIKV).
Moreover, IF tests were negative when antibody to West Nile virus, Toscana virus, Bunyamwera virus
, alphaviruses, phleboviruses and California serogroup viruses was used.