bulked segregant analysis

bulked segregant analysis

A strategy used for rapidly mapping plant genomes, which is suitable for monogenic qualitative traits. When DNA of ten plants are bulked into one pool, all alleles must be present; two bulked pools of segregants differing for one trait will differ only at the locus harbouring that trait.
References in periodicals archive ?
Bulked segregant analysis identifies molecular markers associated with early bulking in cassava (Manihot esculenta Crantz).
Bulked segregant analysis (BSA) was used to identify putative markers tagged the high sugar trait.
The Bulked Segregant Analysis (BSA), in combination with AFLP molecular markers, allowed the identification of DNA polymorphisms in the wild potato Solanum commersonii associated to the resistance gene of the Potato Virus X (PVX).
The DNA marker for the velvet hairiness trait was identified by RAPD technique using bulked segregant analysis.
Simple sequence repeat (SSR) markers were used in three different approaches to search for phytate loci in the Boggs-RR x CX1834-1-2 population: (i) bulked segregant analysis (BSA; Michelmore et al.
Identification of RAPD markers linked the wildfire resistance gene of tobacco using bulked segregant analysis.
Identification of RAPD markers linked to a Rynchosporium secalis resistance locus in barley using near-isogenic lines and bulked segregant analysis.
Subsequently, the selected primer combinations were used for bulked segregant analysis of DNA samples prepared as described from resistant and susceptible individuals for each population.
1997) identified three RAPD markers associated with leaf rust resistance using bulked segregant analysis (BSA).
Another 20 individuals representing a full range of the greenhouse scores, were selected to check the cosegregation with resistance of promising AFLP markers identified by bulked segregant analysis.
The objectives of this study were to (i) identify RAPD markers linked to the CMS-D8 restorer genes by the bulked segregant analysis approach; (ii) convert these RAPD markers into STS markers and make them available to cotton research community; and (iii) study the existence of these markers in other cultivated and wild Gossypium spp.
1995), combined with bulked segregant analysis (BSA) (Michelmore et al.