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buffer2

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buffer2
a double-ended horse shoeing implement designed to be hit with a shoeing hammer. At one end is a broad-bladed chisel face for opening clinches. At the other end is a pointed punch for driving through the shoe nailholes.


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About 100 ng of total DNA was digested with 5U of EcoRI and MseI (New England BIOLABS) in 1X NE buffer2 at 37[degrees]C for 2 h.
 
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