bright field illumination

bright field illumination

A method for lighting a specimen with a solid cone of light. Transmitted bright field illumination (BFI) requires a substage condenser; reflected BFI requires a vertical illuminator.
References in periodicals archive ?
Normal bright field illumination (light passing through the specimen) usually is best for pigment pastes and paint when looking for pigment aggregation, flocculation, and overly large particles.
Under bright field illumination, we found dark and clear DRG neurons are distinctly different, with dark neurons composed of four subpopulations, each with unique numbers and distribution of bright rusty-colored cytoplasmic granules, and statistically significant difference in the soma diameter distribution.
The neurons were examined under bright field illumination on a Zeiss Axiovert 100 microscope and photographed with a Hamamatsu 3-color camera and using Universal Imaging Corp.
When dissociated adult rat DRG neurons are viewed under a compound microscope under bright field illumination, the granules can be seen more clearly, but still, only the single subpopulation of neurons can be identified.
When the DRGs are dissociated and the neurons are examined under bright field illumination under a compound microscope, the difference between the dark and clear neurons becomes strikingly apparent.
The present study was designed to determine whether, under bright field illumination, dissociated adult frog DRG neurons could be categorized into separate populations based upon neuron morphology and neuron diameter.
After 2 days the neurons were fixed in 4% glutaraldehyde, the cover slip with the neurons mounted on a slide, the neurons examined under bright field illumination on a Zeiss Axiovert 100 microscope and photographed with a Hamamatsu 3 color camera and using Universal Imaging Corp.
However, under bright field illumination seven subpopulations of neurons can be identified based solely on the distinct morphologies of the subpopulations.
The DRG was then cut into small pieces and examined under bright field illumination.
The present study was designed to determine whether, under bright field illumination, distinct subpopulations of dissociated adult human DRG neurons could be identified based on neuron morphology and diameter.
All three photographs were taken with bright field illumination (light from below the specimen).