blunt-end ligation

blunt-end li·ga·tion

a reaction that joins two DNA duplexes directly at their blunt ends.

blunt-end ligation

Any molecular technique for joining 2 pieces of blunt-end DNA, such as placing inserts in a cloning vector—e.g., by use of T4 DNA ligase.
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Each was cloned into pPCR-Script by blunt-end ligation and transformed into XL-10 Gold Ultracompetent cells.