blunt-end

blunt-end

(blunt-end),
Refers to double-stranded DNA in which no unpaired bases are turned at the end of the polynucleotide.
References in periodicals archive ?
TurboLigation[TM] Kit enables quick ligation of both blunt-end or cohesive-end DNA fragments in just 5 minutes at room temperature.
Each was cloned into pPCR-Script by blunt-end ligation and transformed into XL-10 Gold Ultracompetent cells.
To keep costs in line, the extrusions used a one-dimensional, blunt-end cut instead of the expected machined face.
The sequences were blunt-end ligated into the EcoR V site of pcDNA3.
The coding sequence of the MT-3 gene was obtained from human proximal tubule cell RNA by RT-PCR, blunt-end ligated into the EcoRV site of pcDNA3.
overhang," blunt-end," and nucleotide pairing design motifs (Woppmann et al.