US 9mm circular needle, 74cm length (or size needed to obtain gauge) Stitch marker Blunt-end
yarn needle Fiberfill stuffing
TurboLigation[TM] Kit enables quick ligation of both blunt-end
or cohesive-end DNA fragments in just 5 minutes at room temperature.
Each was cloned into pPCR-Script by blunt-end
ligation and transformed into XL-10 Gold Ultracompetent cells.
To keep costs in line, the extrusions used a one-dimensional, blunt-end
cut instead of the expected machined face.
The sequences were blunt-end ligated into the EcoR V site of pcDNA3.
The coding sequence of the MT-3 gene was obtained from human proximal tubule cell RNA by RT-PCR, blunt-end ligated into the EcoRV site of pcDNA3.
," and nucleotide pairing design motifs (Woppmann et al.
Samples are tagged with a unique MID using a standard blunt-end